Connective and Muscle Tissues Flashcards
With both Masson and Gomori trichrome procedure, muscle stains:
red
Which of the following methods demonstrate elastic tissue?
a. Verhoeff-van Gieson
b. silver impregnation
c. Gomori trichrome
d. PAS
a. Verhoeff-van Gieson
The first step in most reticulin methods is:
oxidation. First oxidation, then sensitation, silver impregnation, reduction, toning, and removing unreduced silver.
The Verhoeff method differentiates with:
excess mordant
Van Gieson stains collagen what color?
red
Bouin solution functions in the Masson trichrome stains as a/an:
mordant
The components of Van Gieson solution are:
picric acid and acid fuchsin
Gomori aldehyde fuchsin solution contains:
basic fuchsin, hydrochloric acid, and paraldehyde
Most silver stains use what as a toning agent?
gold chloride
The reducing agent in most reticulin stains is:
formaldehyde
Mallory PTAH stain is:
a. polychromic
b. orthochromatic
c. metachromatic
d. apochromatic
a. polychromatic - one stain - two colors.
A good control for reticulin stain is:
liver
Toluidine blue is used to demonstrate which type of cells?
Mast
The component of basement membranes that is usually demonstrated with special stains is:
a. protein
b. carbohydrate
c. laminin
d. collagen
b. carbohydrate
A stain that might be used to demonstrate cirrhosis of the liver is the:
Masson Trichrome
In the Masson trichrome, Biebrich scarlet is removed from the collagen by:
phosphotungstic acid
Iron hematoxylin, rather than aluminum hematoxylin, is usually used to stain nuclei in trichrome procedures because…
subsequent staining solutions are acidic, and will therefore wash off the aluminum hematoxylin
The preferred fixative for the Masson trichrome stain is:
Bouin
Silver impregnation stains for reticulin depend on the formation of which of the following chemical groups?
a. quinoid
b. aldehyde
c. carboxyl
d. amino
aldehyde
The diamine silver complex is formed by a reaction between silver and:
ammonium hydroxide
Sections for the demonstration of the basement membrane should be cut at what thickness?
2 micrometers
The oil red o stain required what type of sectioning?
frozen
The oil red o stain might be used to demonstrate:
a. rhabdomyosarcomas
b. leiomyosarcomas
c. liposarcomas
d. adenocarcinomas
c. liposarcomas
Which of the following is an example of physical staining?
a. toluidine blue
b. verhoeff-van gieson
c. methenamine silver
d. oil red o
oil red o because the tissue absorbs the stain
Fat is chemically fixed and maintained in tissue by:
osmium tetroxide
Plasma cells can be demonstrated with:
methyl green pyronin
When ferric ammonium sulfate is used in a silver stain for reticulin, it functions as the:
sensitizer
Mallory PTAH solution is ripened for immediate use by using:
potassium permaginate
When used in a silver stain for reticulin, phosphomolybdic acid functions as the:
oxidizer
The Verhoeff-van Gieson shows both orange collagen and orange muscle. This can be corrected by:
ensuring that the picric acid solution is saturated, otherwise all tissues will stain the same color
The Masson trichrome stain shows only a faint grayish-pink staining of muscle. This could most likely by prevent in the future by:
using fresh acid-fuchsin-Beibrich scarlet solution
A black precipitate is noted in a periodic acid-methenamine silver stained section that has been on a pathologists desk for about a month, but the stain did not have any precipitates originally. This could be due to:
sodium thiosulfate step may have been omitted. sodium thiosulfate is used to remove unreduced silver so if it was omitted then the silver can reduce in the sun light.
Formalin fixed tissue shows very faded blue staining with the Masson trichrome technique. This could be due to:
left to long in the final acetic acid step. - this can cause overdifferentiation
Microscopic examination of a section stained with the Gomori reticulin stain and counterstained with nuclear fast red shows cloudiness of both the section and slide. This is due to:
inadequate washing after nuclear fast red.
Granular deposition of the silver is seen on the control stained with the Gordon and Sweets reticulin method. One possible reason could be:
The reagents may have been old.
Black precipitation is seen on sections stained with Gomori method for reticulin. This is most likely the result of:
using glassware that was not chemically cleaned
A Masson stain has been requested and the supply of phosphomolybdic acid has been depleted. The best action would be to substitute:
phosphotungstic acid
The connective tissue has failed to stain yellow in the Movat pentachrome stain. One possible reason could be:
safran solution contains water.
The goblet cells in a section of small intestine fail to shoe any blue staining with the Movat pentachrome. This could possibly be a result of:
treating the slides with alkaline alcohol too briefly. The alkaline alcohol converts alcian blue to monstral fast blue, which is insoluble.Complete conversion is necessary otherwise the alcian blue will decolorize during the remainder of the procedure.
