IHC Questions

Question 1

What are the benefits of using a polyclonal antibody?

Answer 1

They are nonselective and can identify multiple epitopes on one anitgen

Question 2

When preparing for an immunhistochemistry (IHC) stain run, what controls are required when staining the patient’s tissue?

Answer 2

Negative, positive and system controls

Question 3

What range of dilutions would you use for the first dilution panel if the antibody data sheet suggested a 1: 50 dilution?

Answer 3

1:10, 1:25, 1:50, 1:100, 1:200

Question 4

What condition is necessary for the secondary antibody in an indirect method?

Answer 4

It must be raised against the IgG of the same species the primary antibody has been raised

Question 5

What was the first source of heat used in heat-induced epitope retrieval (HIER)?

Answer 5

Microwave

Question 6

Which of the following statements are TRUE of a monoclonal antibody?A. Purer than a polyclonal antibody
B. Lower affinity than a polyclonal antibody
C. Are homogeneous
D. Less selective than a polyclonal antibody

Answer 6

A and C

Question 7

What develops as a result of enzyme action involving horseradish peroxidase (HRP) and the enzyme/substrate formation and gives the reaction color in immunohistochemistry (IHC)?

Answer 7

Chromagens

Question 8

What is the one step immunohistochemical (IHC) method called that uses a labeled antibody, like HRP, to react with a specific antigen?

Answer 8

Direct method