Week 9: Dna Replication And Pcr Flashcards
What is the main function of the E. coli replisome?
To facilitate DNA replication.
What are the components needed for PCR?
- Reaction buffer
- Heat-resistant polymerase
- Primers A and B
- Template
- dNTPs
- Mg2+
- Water
What is the typical initial denaturation temperature and duration in a PCR?
95 °C for 5 minutes.
What is the purpose of the reaction buffer in PCR?
It provides the correct pH for the reaction.
Fill in the blank: Taq polymerase is a polymerase derived from _______.
Thermus aquaticus.
What is the elongation rate of Taq polymerase?
1000 bases per minute.
True or False: Taq polymerase has proof-reading activity.
False.
What happens if a newly formed base pair is incorrect during DNA synthesis?
The interactions are disturbed, and the primer cannot be held in the active center.
What is the typical temperature for primer hybridization in PCR?
53 °C for 30 seconds.
What is the role of Mg2+ in PCR?
It is needed for the active center of the polymerase.
What is the purpose of the primer in DNA replication?
To provide a starting point for DNA synthesis.
What is the typical final elongation temperature and duration in PCR?
72 °C for 5 minutes.
What are the three main structural components of the polymerase?
- Palm
- Fingers
- Thumb
What is the consequence of incorrect base incorporation during DNA synthesis?
Incorporation of ~1 mispaired base per 1000 nucleotides.
What type of DNA polymerases can synthesize de novo?
Primase or RNA polymerase.
