WEEK 8: GRAM NEGATIVE COCCI Flashcards
I. General Characteristics
i. Obligate Aerobes
ii. Nonmotile
iii. Oxidase Positive
iv. Catalase positive
v. Gram Negative Diplococci
vi. Capnophilic
vii. Glucose Fermenter
(Except for M. catarrhalis)
Gram Negative Diplococci
acute pyogenic infection, primarily in urethra, endocervix, anal canal, pharynx and conjunctiva.
Neisseria gonorrhoeae
“flow of seed”- urethral discharge was mistaken for semen
Gonorrhea
Venereal disease
Sexually transmitted infection
iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids
Receptors for human transferrin
protective against inflammation
Protein I (porB)
adherence to phagocytic cells and EC
Protein II (Opa)
blocks host serum IgG
Protein III (Rmp) reduction modified protein
attachment to host tissues present in types T1-T2
Pili
T3,T4,T5
avirulent - no pili
protect against phagocytosis
Capsule
Mediate damage to body tissues
Released as outer mebrane fragments “blebs” w/c contain LOS responsible for its virulence
LOS or Endotoxins
cleaves IgA on mucosal surfaces
IgA protease
Most commonly acquired by sexual contact from asymptomatic carrier (primary reservoir)
HPV, C. trachomatis, N. gonorrhoeae
Epidemiology of Neisseria gonorrhoeae
purulent discharge and dysuria
Urethritis in male and Cervicitis in female
AHU strains
arginine, hypoxanthine, uracil
Ophthalmia neonatorum
Conjunctivitis
Urethritis (males ) and cervicitis (female)
PID, sterility, ectopic pregnancy and Fitz-Hugh-Curtis syndrome
Conjunctivitis (Ophthalmia neonatorum)
Clinical Infections of Neisseria gonorrhoeae
Polysaccharide capsule A
pandemics
Polysaccharide capsule B,C
community acquired
Polysaccharide capsule W-135
invasive disease
Polysaccharide capsule Y
pneumonia
enzyme that aids in invasiveness
IgA1 protease
- primary sources are oral secretion/ respiratory droplets (close contact)
- Abrupt onset of frontal headache, stiff neck, fever
Neisseria meningitidis
release of toxins in the blood that
breakdown the walls of the blood vessels
Meningococcemia
hemorrhaging of blood into skin & mucous membranes
Purpura (bruises) and Petechial rash (red spot)
(normal coagulation is disrupted and abnormal bleeding occurs), septic shock, hemorrhage of adrenal glands (Waterhouse-Friderichsen syndrome)
Disseminated intravascular coagulation (DIC)
i. Epidemic meningitis
ii. Meningococcemia
Purpura and Petechial rash
Disseminated intravascular coagulation (DIC)
Waterhouse-Friderichsen syndrome
Clinical Infections of Neisseria meningitidis
- Virulence factor
Attachment to respiratory epithelial cell - Epidemiology
Commensal of the upper respiratory tract - Clinical Infections
i. Localized infections (otitis media and sinusitis)
ii. Lower respiratory tract infections
iii. Systemic infections (endocarditis, meningitis, etc.)
Moraxella catarrhalis
SELECTIVE MEDIUM: Thayer-Martin
INHIBITORY AGENTS: Vancomycin
SUPPRESSED ORG: ?
Gram-positive
CSF, blood, nasopharyngeal swabs and aspirates
N. meningitidis
Genitalia: Urethra (male) and Endocervix (female)
Rectum and Pharynx
N. gonorrhoeae
SELECTIVE MEDIUM: Thayer-Martin
INHIBITORY AGENTS: ?
SUPPRESSED ORG: Gram-negative
Colistin
SELECTIVE MEDIUM: Modified TM
INHIBITORY AGENTS: ?
SUPPRESSED ORG: Swarming of Proteus
Trimethoprim
SELECTIVE MEDIUM: Martin Lewis
INHIBITORY AGENTS: Anisomycin
SUPPRESSED ORG: ?
Yeast
Presumptive identification:
Positive – Neisseria and Moraxella
Oxidase test
SELECTIVE MEDIUM: ?
INHIBITORY AGENTS: Nystatin
SUPPRESSED ORG: Yeast
Thayer-Martin
iv. Incubation
35°C in a 3% to 5% CO2
atmosphere up to __ hrs
72
SELECTIVE MEDIUM:
GC-Lect Agar
INHIBITORY AGENTS: Lincomycin
SUPPRESSED ORG: ?
Gram-positive
SELECTIVE MEDIUM: ?
INHIBITORY AGENTS: Amphothericin B
SUPPRESSED ORG: Yeast
New York City
Small, tan, may be mucoid and convex
N. meningitidis
Small, tan, transluscent and raised
N. gonorrhoeae
Cystine Trypticase Agar Sugar:
glucose positive (yellow); all other are negative (red)
Neisseria gonorrhoeae
Smooth, opaque, gray to white colony may be swept
intact (hockey puck), 48-hr colony - Wagon wheel
M. catarrhalis
What organism is positive in Superoxol test- 30% H2O2?
N. gonorrhoeae
Differentiation of Neisseria species through acid
production from oxidation of carbohydrates
Cystine Trypticase Agar Sugar
what is the indicator of CTA sugars
Phenol red
Cystine Trypticase Agar Sugar:
all tubes negative (red)
Moraxella catarrhalis
Cystine Trypticase Agar Sugar:
glucose and maltose positive (yellow); all other are negative (red)
Neisseria meningitidis
What organism is positive in Superoxol 30% H2O2, MTM, ML, NYC and Glucose
Neisseria gonorrhoeae
For rapid detection of carbohydrate utilization
and production of DNase and beta lactamase
QuadFERM+
What organism is positive in MTM, ML, NYC, Blood agar, Glucose and Maltose
Neisseria meningitidis
this organism is negative in Glucose
Moraxella catarrhalis