WEEK 1: INTRODUCTION TO MICROBIOLOGY Flashcards
The study of organisms and agents too small to be seen clearly by an unaided eye
MICROBIOLOGY
made the earliest microscopic observations between 1625 and 1630 on bees and weevils by the using a microscope probably supplied by Galileo.
Francesco Stelluti
- (1632-1723)-Dutch
- First to described and observe microorganisms accurately.
- Observed these “animalcules” from rain water samples, his own feces and scrapings from his teeth.
- First true microbiologist
Anton Van Leeuwenhoek
- Theory of development of some living forms from non-living matter.
- A “vital force” forms life.
SPONTANEOUS GENERATION
- The theory of development of living forms from another living things.
BIOGENESIS
- Animals could originate from soil.
- Simpler invertebrates could arise from spontaneous generation
Aristotle
- Demonstrate that maggots do not arise from decaying meat
- Experiment
- Jars of Meat with Fine Lace
Francisco Redi
- Experiments on spontaneous
generation. - Nutrient broth boiled without cover, then tightly stoppered the flasks
- Organic matter contained “vital force”
John Needham
- theorized that microorganisms from the air
entered the solution: broth poured in sealed containers heated (-) for microorganisms;
Needham responded by claiming that “vital forces” for spontaneous generation was kept out by the seal.
Lazarro Spallanzani
- Proposed by Rudolf Virchow in 1858- a German scientist challenged spontaneous generation with the concept of Biogenesis i.e. living cells arise from pre-existing living cells
- The theory of development of living forms from another living things.
Biogenesis
Who proposed BIOGENESIS?
Rudolf Virchow
- He observed that no growth occurred in a flask that contained nutrient solution after allowing the air to pass through the heated tube.
- Stated that yeast cells are responsible for the conversion of sugar to alcohol.
Theodor Schwann
- Firmly Disprove the spontaneous generation doctrine (1861) and resolved all arguments.
- Demonstrated through experimentation that
microorganisms are present in the air and can contaminate sterile solutions BUT that air itself does not create microbes. These findings became the basis of ASEPTIC TECHNIQUES.
Louis Pasteur
He is also known as the Father of Bacteriology.
Louis Pasteur
Process which prevented wine from turning sour.
Wine was heated at a
temperature which will kill
microorganisms responsible for the sour taste
FERMENTATION
Responsible for fermentation
YEAST
Process which involved milk
PASTEURIZATION
- He showed that dusts carry germs that could contaminate a sterile broth
- Proposed the use of moist heat for 3 consecutive days to eradicate vegetative cells and endospores now known as Tyndallization.
John Tyndall