Week 4_Cell division_eukaryotic cell cycle Flashcards
what is a chromosome?
condensed molecules of DNA associated with proteins
a chromosome contains a particular set of genes
describe chromosome nomenclature:
you can have a 1-chromatid chromosome
you can have a 2-chromatid chromosome
what is a centromere?
an area of repetitive DNA sequence in a chromosome
what holds the 2 chromatids together in a 2-chromatid chromosome?
cohesion proteins
during cell division, ___ attach to the centromere of each sister chromatid
2 types of proteins
- cohesion proteins
- kinetochore proteins
what are cohesion proteins?
holds the sister chromatids together after DNA replication (inner part of the centrosomes)
what are kinetochore proteins?
outer part of the centromeres
the site of microtubule attachment during cell division
what are telomeres?
repetitive sequences of DNA that protect and stabilize the ends of chromosomes
each time a cell divides, the telomeres become ___
shorter
each time a cell ___, the telomeres become shorter
divides
once the telomeres become too short, the cell…
can no longer divide
telomere length can be maintained by the enzyme ___
telomerase
most cells in the body stop producing telomerase after ___
embryonic development
in ~90% of human cancers, telomerase production has been ___
reactivated
each human cell contains ~___ of DNA
2 meters
the nucleus of a human cell, which contains the DNA, is only ~___ in diameter
6 micrometers
how does DNA coil?
DNA has a (-) charge
histone proteins have a (+) charge
146 bp of DNA wraps around 8 histone proteins to form a nucleosome
nucleosomes coil into a selenoid
selenoids are compacted further into supercoils
the level of DNA compaction changes (it’s dynamic)
what is chromatin?
chromosomal DNA associated with proteins
what is a nucleosome?
the basic unit of chromatin
how is a nucleosome made?
146 bp of DNA wrapped around 8 histone protiens
describe the DNA compaction levels
DNA wraps around histone proteins to form nucleosomes
nucleosomes coil into selenoids
selenoids compact further into supercoils
during lower level of DNA compaction, individual chromosomes ___ distinguishable
are not
during higher level of DNA compaction, individual chromosomes ___ distinguishable
are