WEEK 3: DNA/Molecular RBC Typing

Question 1

list the advantages of molecular rbc typing. (4)

Answer 1

• Can provide a full genetic type for highly prevalent antigens
• Simple 3 step procedure giving results in under 3 hours
• Most technology (PCR cyclers) already present in H&I laboratories* Specialised Reader

Question 2

what is the principle of the TaqMan Probe PCR-SSP?

Answer 2

DNA from cell
primer will bind and amplify, transcribe along, probe extends and releases quencher molecule??????????????????????????

Question 3

what are some advantages of the TaqMan Probe PCR-SSP? (7)

Answer 3

• Most useful in multi-transfused and AIHA cases
• Results can be obtained the same day
• Reproducible, Low fail rate
• Good concordance with serological typing results
• Simple to use and not labour intensive
• No significant wastage
• Direct download of results

Question 4

what are some disadvantages of the TaqMan Probe PCR-SSP? (4)

Answer 4

• Not suitable for urgent cases
• Not as fast as serological typing (see urgent cases above)
• Not suitable for rare genotypes – require sequencing
• ABO – simple serologically but complex genetically(not performed on this assay).