W9 Assisted Repro Techniques Flashcards
AI process
Cellection of semen using AV
Process semen - chill or freeze
Storage/ transport too females in oestrus
Inseminate
Advantages of AI
Genetic progrress
No need to own male
Reduses disease transmittion
Semem from OS used
Disad of AI
Costly
Specialised facilities
How to do it
Cow - Transcervica, guided per rectum
Mare- Transcervical, guided per vagina
Ewe- Vaginal, transcercial, transabdominal
Bitch- cranial vagina or transcervical
Synchronisation Technique on cattle
PGF programs - must be day 6-17 of cycle, wont work if not cycing, PGF causes luteolysis of CL
Progesterone programs - supress GnRH = no ovulation= have in for 14 days, pull out = synchronised
GnRH + PGF
WHat is ET
Removal of preattachement embryos from repro tract of donor and transferring them into athe repro tract of recipient
ET application
Exploit genetic potential
Import and export
Enhance repro in endangered species
Freeze for sufuture generations
Steps of ET
Selection of donor and recipient
Synchronising the cycle of donors and recipients
Super ovulation of donor
AI of donor
Recovery embryos from donor
Grading and maintenance of viable embryos in vitro
Transfer to recipient
Best preg of ET synchronisation
recipient in oestrus 36 hrs before to 12 hrs after the donor
Recooment 8 recipients per donor cow
Step 2 Super ovulation
Hyerstimulaiton of gonadotrophins = more follicles to become dominant and ovulate (FSH)
Step 1
Synchronisation of oestrus cycle to ensure reciepint uterus is ready
Prostaganlins
CIDR
Synchronation programs
Embryo recovery
Day 6-8
Foleys catherer used for flushing
Search for them and grade once recovered
Maintenance of embryos
Room temp for several hours
Change holding media every 2 hours ph 7.1-7.5
size of embryo 150-180 micron
IVF vs ET
Proglongs reproductive life of a cow that do not respond to super ovulaiton
Can be applied during diff stages of repro- up to 3 months
However decreased preg rates, more male calve
Increased birth weights
