W4 microscopy

Question 1

light source

Answer 1

radiant field diaphragm adjusts the intensity of brightness and it is located above the light source controls diameter

Question 2

condenser assembly

Answer 2

located above light source and just under slide.
the condenser acts to focus light onto the slide on the stage. an adjustable aperture diaphragm controls the angle of the cone of light reaching the specimen the alignment is what the kohler illumination is

Question 3

stage

Answer 3

where slide placed for viewing.

Question 4

slide controls

Answer 4

move stage in a horizontal plane

Question 5

coarse focus knob

Answer 5

moves stage up and down

Question 6

nose piece

Answer 6

holds objectives

Question 7

objectives

Answer 7

different settings 10x 40x and 100x magnify the specimen

Question 8

parfocal

Answer 8

nosepiece and objective assembly that allows different objectives to focus at the same place. you won’t need to move coarse knob only fine focus knob

Question 9

body tube

Answer 9

enclosed tube that physically separates the objective from optical lens

Question 10

oculars

Answer 10

located in adjustable binoculars. provide 10x magnification

Question 11

primary magnification and ocular lens

Answer 11

this is done by the objective lens and the object is seen as real, magnified and flipped. and this image coming from the objective lens is what the ocular lens magnifies which creates a virtual, magnified and upright image

Question 12

total magnification of microscope

Answer 12

multiply magnification of two lenses

Question 13

refraction

Answer 13

light bends as it passes through mediums of differing densities the refractive index measures the density of a medium

Question 14

immersion oil

Answer 14

immersion oil used to prevent loss of light due to refraction and ensure adequate light is collected y smaller diameter lens

Question 15

resolving power

Answer 15

ability of a lens to discern fine detail and produce a clear image of the specimen

Question 16

numerical aperture na

Answer 16

mathematical expression of the ability of a lens to gather light
cone of light leaving the condenser lens, through the slide and entering the objective lens aperture. a narrow cone has a low na and a wider cone has a high na
** higher the numerical aperture the better the resolution
**numerical aperture of the condenser should match the NA of the objective in oder to obtain the best resolution

Question 17

kohler illumination

*

Answer 17

aligns the components of a microscope to provide bright even illumination and optimal resolution

• radiant field diaphragm *controls amount of light going into condenser
• height adjustment-assembly adjusted to focus light onto specimen
• adjustable condenser aperture diaphragm -match the na of the objective to improve resolution