extra stuff to know for test from all weeks

Question 1

how to get into kohler

Answer 1

1. close radiant field diaphragm
2. open condesner aperture cimpletely
3. raise condesner to highest position
4. looking into microscope lower condenser and stop when diaphragm image is in sharp focus- crisp edges
5. center image using screws
6. open radiant field diaphragm until view field is completely filled with light-stop when light is just outside field.
7. remove ocular and close condenser until only 70% is of light coming in
8. adjust brightness to personal preference

Question 2

how to set up microscope

Answer 2

turn microscope on
put everything to middle setting
ensure nosepiece in park
clean oculars
clean lens in increasing order
adjust eye distance 
raise condenser to highest position

Question 3

what are volumetric pipettes used for

Answer 3

accurate measurement of aqueous fluids such as controls or standards

Question 4

what are mohr pipettes used for

Answer 4

used to measure fractions of a total volume-same solution into multiple test tubes
not as accurate as volumetric pipettes
used to measure reagents or water
not accurate enough for samples or standards

Question 5

what are semiautomatic pipettes and what are they used for

Answer 5

they are MLA pipettes, eppendorf pipettes and hamilton pipettes and they are used to measure small volumes of liquids in microlitres

Question 6

use of biological safety cabinet class 2A2

Answer 6

uv off drain closed
-flourescent light on
-run blower for 5 minutes and record pressure gauge
-ensure sash is open no more than 10 inches
-disinfect all interior surfaces of BSC
-place everything you need in in cabinet
-wear PPE use absorbent pad for spills
-keep area clean
keep intake grills free of obstacles
work 10-15 cm inside of the cabinet
-limit arm movements in order to not disrupt air flow
-wait one minute after you have moved arms into the cabinet veer you start to work to let airflow stabilize
-dont use flames

Question 7

what to do when done using the bunsen burner

Answer 7

• ensure containers are closed and covered outsides and disinfected before properly disposing of all contaminated materials
• disinfect with cavicide or other appropriate disinfectant that will not damage metal
• allow cabinet to purge by leaving the blower on for at least 5 minutes after cleaning.
• remove gloves and dispose of them in the BSC

Question 8

How to use chemical fume hood

Answer 8

• fluourescent light off
• check are vents free of obstruction
• make sure sash at proper height
• make sure hood is working properly