W3 pipette info - lab glassware Flashcards
volumetric pipettes
always calibrated td a single volume
used to make aqueous fluids such as controls or standards
mohr pipette
not as accurate as volumetric
measuring reagents or water
not calibrated to tip therefore pipette not drained past last mark on stem
used to measure fractions of a total volume s-saves time
serological pipette
not suitable for analytical work drains fast large last drop blown out used for lots of dilutions allowing delivery of diluted sample without leaving the sample remaining in tip used for measuring thick fluids due to large orifice ** do not wipe wit tissue ** blow out last drop
pasteur pipettes
not accurate
develop good technique and avoid splatter creating aerosol production
semiautomatic pipettes
small volume
disposable tip
very accurate
** hold vertically
**smooth depression and release of plunger
** depress plunger before insertion - if air bubbles are in tip, discard tip
** do not release plunger while in receiving vessel it may draw up solution
mla pipette
single or adjustable volume
yeah
eppendorf or hamilton pipettes
two stops
depress to first stop then pick up sample and go to second stop when delivering
read the care and maintenance at the end of lab equipment
do it for real or eventually make flashcards