W3 pipette info - lab glassware Flashcards

1
Q

volumetric pipettes

A

always calibrated td a single volume

used to make aqueous fluids such as controls or standards

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2
Q

mohr pipette

A

not as accurate as volumetric
measuring reagents or water
not calibrated to tip therefore pipette not drained past last mark on stem
used to measure fractions of a total volume s-saves time

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3
Q

serological pipette

A
not suitable for analytical work
drains fast 
large
last drop blown out 
used for lots of dilutions allowing delivery of diluted sample without leaving the sample remaining in tip 
used for measuring thick fluids due to large orifice 
** do not wipe wit tissue
** blow out last drop
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4
Q

pasteur pipettes

A

not accurate

develop good technique and avoid splatter creating aerosol production

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5
Q

semiautomatic pipettes

A

small volume
disposable tip
very accurate
** hold vertically
**
smooth depression and release of plunger
** depress plunger before insertion - if air bubbles are in tip, discard tip
** do not release plunger while in receiving vessel it may draw up solution

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6
Q

mla pipette

A

single or adjustable volume

yeah

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7
Q

eppendorf or hamilton pipettes

A

two stops

depress to first stop then pick up sample and go to second stop when delivering

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8
Q

read the care and maintenance at the end of lab equipment

A

do it for real or eventually make flashcards

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