W3 Proteins structure and synthesis Flashcards
What are the main functions of proteins? (4)
Carrying out enzymatic functions
Generating movements of cells
Carrying signal from cell to cell and within individual cells
Providing support to cells and tissues
What are proteins made up of?
Linear chains of amino acids arranged in a 3D structure
What does the function of a protein depend on?
A protein’s amino acid sequence
What are ISD’s- Intrinsically disordered proteins? (not mandatory)
They are proteins that have lost their native, functional folding, resulting in an insoluble aggregation in many organs, causing many diseases.
How many amino acids are there?
20
What do all amino acids have in common? (structure)
What is different?
C, COOH, H, NH2 and different R group (specific for each amino acid)
What are the 3 phases of (DNA) transcription called?
Initiation
Elongation
Termination
What occurs during initiation?
(transcription)
- RNA polymerase binds to a specific sequence of DNA (promoter).
- RNA polymerase separates the double strands and uses a single strand as a template.
- 2 initial RNA nucleotides are joined together to begin RNA strand synthesis.
What occurs during elongation (transcription)?
RNA polymerase moves along the DNA coding sequence in the 5’ to 3’ direction. It melts duplex DNA whilst it adds ribonucleotides to the growing RNA
What happens during Termination?
(transcription)
The RNA polymerase encounters a stop codon, this releases the RNA strand
The newly RNA transcript is a precursor of mRNA
How can a protein and a peptide be classified separately?
Peptide= Short chain (<50 amino acids)
Protein= Longer chain (>50 amino acids), also referred to as polypeptides
What are the 3 steps of RNA processing?
- Capping at the 5’ end
- Polyadenylation at the 3’ end
- Splicing
What is capping at the 5’ end?
What linkage?
A 5’ cap (7-methylguanate) is added to the ribonucleotide at 5’ by an usual 5’-5’ triphosphate linkage
What is polyadenylation?
A repetition of poly-A is added to the 3’ end
What are the 3 steps of translation?
What are the steps? (simple)
Initiation- The ribosome gets together with the mRNA and the first tRNA
Elongation- Amino acids are brought to the ribosome by tRNAs and linked together to form a chain
Termination- The finished polypeptide is released to go and do its job in the cell.