Viruses Flashcards

Test 4

1
Q

T/F: viruses are living organisms

A

F

Lack nucleus, mitochondria, cell membrane, ribosomes

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2
Q

We want to ______ an active virus

A

inactivate

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3
Q

Viruses are either _____ or ______

A

Active or inactive

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4
Q

Why are viruses considered intracellular parasites?

A

They cannot multiply –> have to invade a specific host cell

after invasion, instructs host cell to make and release new viruses

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5
Q

Differentiate between the lysogenic cycle in the lytic cycle
Give examples

A

Lysogenic cycle: virus slowly pinches off the cell
-lasts years/decades
Ex) HIV

Lytic cycle: Burst host cell r/t acute infections
-clears out fast
Ex) Flu

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6
Q

T/F: Virus have no effects on animals & plants

A

F

Plants effected the worst actually

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7
Q

What are the 3 main parts of a virus?

A
  1. external coating (outer coat/Capsid): repeating proteins that bind & form a capsid
  2. core containing nucleic acids (DNA/RNA)
  3. spikes: target specific cell that it’s going to infect
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8
Q

Are DNA strands mostly single or double stranded? RNA?

A

DNA: double
RNA: Single

But DNA/RNA could be either

Single stranded DNA = Parvo

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9
Q

What does retrovirus mean? What are examples

A

Starts off as RNA but changes to DNA & then put into nucleus of host cell
-All these viruses can be reactivated

Ex) HIV, Herpes, HPV16/18

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10
Q

T/F: retroviruses can be reactivated

A

T

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11
Q

What is the enzyme used in retroviruses to convert RNA to DNA?

A

Reverse transcriptase

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12
Q

T/F: all viruses have an antiviral therapy

A

F

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13
Q

What is the difference between a naked & enveloped virus?

A

Naked: Has capsid, nucleic acid, & spike

Enveloped: In addition to things listed naked virus; has an enveloped “outer cell membrane”

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14
Q

What does the outer cell membrane come from in an enveloped virus? What type of cycle do you see this in?

A

part of the cell host that it has infected

lysogenic cycle

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15
Q

What is the viral spike in HIV? What does it target?

A

GP120

CP4(+) proteins on T cells & marcophages

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16
Q

What are the 5 steps for Viral replication? Describe them. Why are they important?

A
  1. Absortion: attachment of virus to host cell; viral spike recognizes & attaches to protein target on host cell
  2. Penetration: entry of virion/genome into host cell
    -some viruses have to uncoat (shed its outer membrane) to enter the cell or they inject the nucleic acid directly
  3. Synthesis: new nucleic acid, capsid proteins, & other viral components (ex. reverse transcriptase)
    This is where the host cell now becomes the factory for making new viruses
  4. Maturation: assembly of newly synthesized viral components into complete virion
    -moves virus to cell surface
  5. Release: departure of nre virion from host cell

Antivirals target these steps

17
Q

What are some viral infections that only occur in certain regions?

A

Dengue fever
rift valley fever
yellow fever

18
Q

Why are viruses difficult to design a vaccine or antiviral for?

A

Mutate at rapid rate

19
Q

Drugs: Acyclovir

A

Class: Antiviral; Chain terminator

Prototype

MOA: inhibit DNA synthesis
-very similar structure to DNA but missing part of sugar & hydroxyl group “Chain termination”
-another base cannot be added

Uses: Herpes, HSV, VSV
-reduces viral shedding

SE: mild; Renal insufficiency related to crystalization when given IV
-Can be reversed after d/c with hydration

20
Q

What causes AIDS? How do you usually die from AIDS?

A

HIV

Opportunistic infection d/t failure of the immune system

21
Q

Drugs: Azidothymidine, Lamivudine

A

Class: Antiviral

MOA: Azidothymidine: inhibits reverse transcriptase
-Lamivudine: inhibits DNA polymerase & reverse transcriptase

Azidothymidine is primary

Uses: HIV (Part of HAART cocktail)
-reduces viral shedding

22
Q

Drugs: Cabotegrovir (PrEP)

A

Class: Antiviral
MOA: Blocks integration/absorption

Take this before exposure to HIV to prevent from getting infected in the first place

23
Q

Drugs: Recombinent Interferon

A

Class: Antiviral
MOA: Activates MHC-1 receptors on cell surface that target CD8(+) T cells (Killer cells)
- CD8+ T cells fire cytokines at host cell to kill off host cell

We can produce this endogenously within the host cell

24
Q

What are the killer cell? What do they do?

A

CD8+ T cells

Once on the MHC-1 receptor, they fire cytokines at virus host cells

25
Q

How many different types of interferons are there?

A

20

26
Q

Who is supposed to get their flu vaccine & how often?

A

Above 6 months old

every year

27
Q

Differentiate between the 2 types of flu

A

Type A: Are a combo of 2 different subtypes
Hemagglutinin (role in absorption): H1, H2, H3 or
Neuraminidase (role in release): N1, N2

-Effects everything
-Moderate to severe cases

Type B: milder cases
-Only effects humans & seals

28
Q

What is the most common target site for antivirals with the flu?

A

Neuraminidase which has a role in release

29
Q

Drugs: Oseltamivir phosphate (Tamiflu)
Baloxivir marboxil (Xofluza)

A

Class: Antivirals - Flu
MOA: Tamiflu: targets Neuraminidase
Xofluza: targets polymerase enzyne

Need to take within 1st 48 hrs of symtpoms

Xofluza: not recommended for pregnant/breastfeeding or complicated illness pts

30
Q

Drugs: Zanamivir (Relenza)

A

Class: Antivirals - Flu
MOA: targets Neuraminidase

Powder inhalant
Not for people with COPD or asthma

Have to be taken when you’ve been exposed to the flu BEFOREEEE symptoms occur

31
Q

What are different types of Coronaviruses?

A

SARS
MERS
COVID-19

32
Q

Drugs: Paxlovid, Remdesivir, COVID Mabs, Dexamethasone

A

Class: Antivirals - Covid

MOA: Remdesivir: chain terminator

-COVID Mabs: Block absorptoon of COVID into host cells

-Dexamethasone: Suppress immune system to suppress cytokines to prevent cytokine storm in severe covid cases

-Paxlovid- 2 antivirals combined (experimental medication

33
Q

What is the #1 CDC recommendation for COVID?

A

Vaccination