VIROLOGY 5 - CULTIVATION AND DETECTION OF VIRUSES Flashcards
why can viruses not grow in standard microbiological media?
they cannot replicate by themselves
cannot grow in broth or on agar plates
how are viruses cultured?
inside living host cells, and there are three types
- media consisting of mature organisms (bacteria, plants or animals)
- fertilized eggs
- cell cultures
how are viruses cultured in bacteria
how are viruses cultured in plants and animals
what are the advantages of using embryonated egg cultures?
how is the virus injected in the egg for cultures?
what are the advantages of cell cultures
how are viruses introduced to the cell culture?
you get primary and continuous cell lines
what is a primary cell line?
what is a continuous cell line?
what are cytopathic effects (CPE)?
how does a plaque assay work and what does it mean
renato dulbecco won a nobel prize for setting up the technique
how is a plaque formed by a lytic virus
what is multiplicity of infection (MOI)?
what is the poisson distribution
describes the number of virions that each cell receives at different MOI
different MOI are used for different experiments
what is a focus forming assay (FFA)?
