CONTROL OF MICROORGANISMS 1 Flashcards
where is it that microorganisms are the most present?
as you go to more biological/nutrient rich things there are more microorganisms
the microorganisms on our skin are not disease causing, they even protect us
where must microbial control in the environment happen?
inanimate surfaces (operating tools, beakers)
living tissues (operations)
food water sources and other liquids
airborne biohazards (sneezing, coughing)
how does food poisoning and spoilage happen
E.coli is famous for meat
salmonella and e.coli can contaminate onions
E.coli O157 is very versatile, can grow in a lot of environments, particularly in hamburgers
medicine and antimicrobial therapy
- pre op and post op antibiotics
- the air is filtered
- nosocomial=hospital acquired
biohazard control
industrial companies that specialise in the breakdown of waste
they are incinerated to destroy everything
biohazard control and public health outbreaks
ebola, marburg
dengue
norovirus
tuberculosis
biohazard control and public health epidemics and pandemics
influenza
bubonic plague
HIV/AIDS
SARS-Cov2
smallpox
during the plague, first evidence of quarantines happening (isolate infected individuals)
influenza: first use of masks
microbial control in water
potable and filtered water
waste and water management
facilities filter and clean water
what are the levels of microbial control?
the first three deal with vegetative cells (cells that grow and do nothing)
the first three cannot deal with spores
sterilisation kills spores as well
use these techniques together, not one or the other
example: first sanitise and then disinfect
sterilisation is done by heat, chemicals, radiation or mechanically
difference between bactericidal vs bacteriostatic
measuring how efficient a killing agent is
population death is logarithmic
D value= time to go down one log
what are factors that influence microbial control?
different organisms require different conditions to kill them
bacterial cells are easiest to kill, spores are the hardest
ways of microbial control in a fumehood
what are physical methods of microbial control
how does an autoclave work
if you pack it full of stuff, it’ll be less efficient
how does pasteurization work
what are food spoilage signs
most often though, you cannot tell, and you end up being sick
how does UV radiation microbial control
how does gamma radiation microbial control work
