viral gastroenteridis

Question 1

when is specific viral Dx helpful

Answer 1

outbreak
severe disease
immunocommpromised

rt pcr or multiplex pcr

Question 2

TX

Answer 2

Rehydration and eletrolyte correction

Question 3

Norovirus

Answer 3

ssRNS naked
viral encoded protease
short on short off
cruise ships, hospitals nursin ghomes
ASX shedding
10-100 infectious dose
fecal oral

*Has replaced rotavirus as #1 in areas where rotavirus vaccine in use

Question 4

Rota virus

Answer 4

dsRNA in segments
issue in developin gworld
winter spring
non-enveloped wheel virus

VP-6—A: frequent human infection; most important human group

human and animal

NSP4, a secreted non-structural protein!

Question 5

rota virus gobbstopper

Answer 5

Outer capsid layer: acid stability
Composed of VP7 with VP4 spikes: induce neutralizing antibody

Inner capsid layer
Contains VP6: major rotavirus group antigen

Innermost core: VP2

Question 6

NSP4, a secreted non-structural protein!

Answer 6

Diarrhea has been induced in research mice and rats by inoculation with NSP4
Destabilizes membranes, leading to cell death
Mobilizes intracellular Ca++ activating signal transduction pathway to transport from ER
Cl- secretion and water follows Cl-
? Activation of enteric nervous system

Question 7

is the single most important cause of severe infantile gastroenteritis worldwide

Answer 7

Rotavirus

Question 8

what negative thing is rotavirus vaccine associated with

Answer 8

intussiception

Question 9

rotavirus vaccines

Answer 9

RotaTeq (RV5): Pentavalent live bovine rotavirus vaccine
Contains outer capsid proteins of 5 human RV strains made by reassortment with bovine RV genome segments
75% protective against disease, 98% protective against severe disease
Oral, 3 doses (2, 4, 6 months)

Rotarix (RV1): Monovalent live human rotavirus vaccine
Provides cross-protection against other strains
85% protective against severe disease
Oral, 2 doses (2, 4 months)

Question 10

Adenovirus

Answer 10

Non-enveloped, icosahedral double stranded DNA virus
Most commonly known to cause URI, pharyngitis, conjunctivitis, pneumonia, hemorrhagic cystitis
Serotypes 40, 41 can cause gastroenteritis

Often asymptomatic
Watery diarrhea, then vomiting lasting 5-12 days
Less dehydration than rotavirus
Low-grade fever
Re-infections can occur
Can cause persistent, severe infection in immune compromised hosts

can be grown in culture