VEST 1 Flashcards
The addition of a 5’ end 7-methyl G cap to mRNA protects it from degradation by _________.
5’ exonucleases
The 5’ end 7-methyl G cap enhances the _________ of the 5’ most exon.
splicing
the ________ is necessary for targeting mRNAs for export to the cytoplasm through the nuclear pores.
5’ end 7-methyl g cap
In the cytoplasm, the 5’ end 7-methyl G cap interacts with ___________ to form a closed loop structure.
poly(A) tail binding proteins (at the 3’ end)
The closed loop structure formed by the 5’ end 7-methyl G cap and poly(A) tail binding proteins recruits the ________ for translation.
ribosome
RNA methylation to form N6-methyladenosine (m6A) in pre-mRNA is an abundant internal modification that controls ____________.
gene expression
The process of RNA methylation to form N6-methyladenosine (m6A) is referred to as the ______________.
epitranscriptome
m6A is found in mRNAs involved with specific _________ and __________.
developmental processes, cell differentiation
Most m6A sites are ________ and are similar in different tissues.
constitutive
m6A is enriched in the __________ and near _______, but also occurs in the _______ and internally.
3’ UTR and near stop codons, 5’UTR
The presence of a __________ is a strong inducer of m6A addition in the transcribed mRNA.
long internal exon
m6A addition occurs in the ________ during __________.
nucleus, transcription
The m6A writer complex adds the m6A _________.
co-transcriptionally
The m6A writer complex contains _________ and ________ proteins.
METTL3, METTL14
m6A erasers are mostly found in the _________.
nucleus
The main m6A eraser is __________.
ALKBHS
In the nucleus, the m6A binds “________”.
reader proteins
In the cytoplasm, other “reader proteins” affect ____________.
mRNA stability, localization,
and translation of the mRNA
_________ is the regulated removal of intron sequences from pre-mRNA.
Splicing
Splicing involves the simultaneous joining (________) of exon sequences together.
ligation
Exon sequences contain the _______________________.
5’ untranslated regions (UTR), protein coding regions, and the 3’ UTR
________ sequences are exported from the nucleus.
Exon
__________ is catalyzed by RNA.
Splicing
Pre-mRNA splicing uses the same chemistry as __________ but needs more help.
group 2 self-splicing