MILLAY 2 Flashcards
The isolation of fusion factors using a cell-free system led to the discovery of _______ and eventually _______.
NSF, SNAREs
In an in-vitro vesicle fusion assay (FRET), fusion _______ the dyes, allowing fluorescence.
dilutes
SNAREs constitute the minimal machinery for __________________.
membrane fusion
The release of synaptic vesicles involves _______.
NSF
Specificity in vesicle targeting is provided by _______ and _______ proteins.
SNAREs, Rab
SNARE-mediated membrane fusion resembles _______.
viral fusion
What is the name of the gene discovered in the study of C. elegans epithelial cell fusion?
EFF-1 (Epithelial Fusion Failure)
True or False: Studies have shown that Eff-1 is sufficient to fuse cells that normally do not fuse.
True
What is the role of Myomaker in muscle cell fusion?
Myomaker-expressing fibroblasts fuse to muscle
The study of Myomaker led to the identification of additional ______________.
fusion factors
Gm7325 (aka Myomerger) induces _______________.
fusogenicity
Why don’t membranes spontaneously fuse?
Membranes do not spontaneously fuse because proteins must overcome phospholipid repulsion forces.
Provide three examples of traditional fusion proteins.
HIV, VSV-G, SNAREs
Traditional fusion proteins interact between membranes and undergo a __________________ that brings membranes in close proximity.
conformational change
A physical interaction is required for the function of _________ fusogens.
classical
