Miller (1&2) Flashcards
The concentration of GTP in the cell is _______ times greater than GDP, which is why GDP falling off the GPCR typically leads to GTP loading onto the alpha subunit.
10
What are the three subunits of a heterotrimeric G protein?
Alpha, beta, gamma
All GPCRs have ____ transmembrane domains.
7
True or False: The ligand for a GCPR always binds directly to the amino-terminus.
False; while the ligand can bind to the amino-terminus, they often bind further into the membrane and contact multiple transmembrane domains.
The DRY motif in a GPCR is found at the end of transmembrane domain ____.
3
In an inactivated state, the ionic-lock is considered intact due to the DRY motif interacting with a ________(residue) in TMD 6.
glutamate (E)
___________ basically function as exchange factors.
GPCRs
In a/an _________ model, ligand binding to the inactive receptor converts the receptor to the active form.
induced fit
In a/an _________ model, the ligand stabilizes the active form of the receptor, which naturally moves between active and inactive forms.
conformational selection
_________ on tyrosine residues starts the signaling in Tyrosine Kinase Receptors.
Phosphorylation
PXXP domains can bind to _____ domains.
SH3
PH domains bind to __________.
phospholipids
SH2 domains bind to __________.
phosphotyrosine
PTB domains bind to _________.
peptides
True or False: All PTB domains bind to all phosphotyrosines.
False, there’s specificity
