MILLAY 1 Flashcards
The estimated density of TM proteins in the ER and Golgi is approximately ___________/μm3.
30,000
Three well-characterized coated vesicles include clathrin, ______, and COPII.
COPI
The basic steps of vesicle transport include ___________, transport, and fusion.
fission
Protein trafficking requires the generation of regions of intense ___________ ___________ in a reversible fashion.
membrane curvature
A type of protein that assists with membrane bending is called a ___________ protein.
BAR
The Golgi is found in a “___________” location in most eukaryotic cells.
juxtanuclear
The cis and trans faces of the Golgi are named relative to the ___________ or ER.
nucleus
Two major functions of the Golgi are protein and ___________ modifications and sorting.
lipid
Two types of exocytosis are constitutive and ___________ secretion.
regulated
The endocytic pathway leads from the plasma membrane to ___________.
lysosomes
Endocytosis controls the ___________ of plasma membrane proteins.
localization
The import of ___________ involves LDL receptors being returned to the plasma membrane.
cholesterol
Some internalized receptors are targeted to ___________ and degraded.
lysosomes
Early endosomes are ___________.
heterogenous
___________ receptors (red) constitutively cycle between endosomes and the plasma membrane.
Transferrin
___________ receptors (green) are down-regulated by endocytosis followed by degradation in lysosomes.
Opioid
Endocytosis of both transferrin and opioid receptors initiates in the same ___________- ___________ pits.
clathrin-coated
___________ receptors are sorted to the recycling endosomes.
Transferrin
___________ receptors are sorted to late endosomes/lysosomes.
Opioid
___________ transporters are stored in recycling endosomes for fast mobilization.
Glut4
AP2 binds to cargo receptors in ___________-rich membrane, then recruits
PI(4,5)P2
Clathrin provides support for the best-studied route of ___________.
endocytosis
The steps in endocytosis include marking the site, ___________ clathrin, bending the membrane, scission, and uncoating.
recruiting
Molecules involved in marking the site for endocytosis include ___________.
Phosphatidylinositols (PIs)
Molecules involved in recruiting clathrin include ____________________.
AP2, PI, cargo, AP180, Epsin
Molecules involved in bending the membrane include ___________ domain proteins and ___________.
BAR, Epsin
The molecule involved in scission is ___________.
Dynamin
Molecules involved in uncoating include ___________, ___________, and ___________.
Hsc70, Auxilin, Synaptojanin
AP180 recruits clathrin through ‘___________ ___________’ interactions.
fishing line
The golgi is the sorting station for the ___________ pathway, while the endosome is the sorting station for the __________ pathway.
exocytic, endocytic
___________ recognizes membrane regions rich in PI(4,5)P2, inserts into the membrane, bending it, and then recruits AP2 and clathrin.
Epsin
Dynamin, a _____ase, pinches off the clathrin-coated vesicle (CCV) from the plasma membrane.
GTP
True or False: COPI vesicles go from ER to Golgi, while COPII vesicles go from Golgi to ER.
False; COPI - Golgi to ER, COPII - ER to Golgi
_______-microscopy can be used to measure and observe membrane binding.
TIRF
