MILLAY 1

Question 1

The estimated density of TM proteins in the ER and Golgi is approximately ___________/μm3.

Answer 1

30,000

Question 2

Three well-characterized coated vesicles include clathrin, ______, and COPII.

Answer 2

COPI

Question 3

The basic steps of vesicle transport include ___________, transport, and fusion.

Answer 3

fission

Question 4

Protein trafficking requires the generation of regions of intense ___________ ___________ in a reversible fashion.

Answer 4

membrane curvature

Question 5

A type of protein that assists with membrane bending is called a ___________ protein.

Answer 5

BAR

Question 6

The Golgi is found in a “___________” location in most eukaryotic cells.

Answer 6

juxtanuclear

Question 7

The cis and trans faces of the Golgi are named relative to the ___________ or ER.

Answer 7

nucleus

Question 8

Two major functions of the Golgi are protein and ___________ modifications and sorting.

Answer 8

lipid

Question 9

Two types of exocytosis are constitutive and ___________ secretion.

Answer 9

regulated

Question 10

The endocytic pathway leads from the plasma membrane to ___________.

Answer 10

lysosomes

Question 11

Endocytosis controls the ___________ of plasma membrane proteins.

Answer 11

localization

Question 12

The import of ___________ involves LDL receptors being returned to the plasma membrane.

Answer 12

cholesterol

Question 13

Some internalized receptors are targeted to ___________ and degraded.

Answer 13

lysosomes

Question 14

Early endosomes are ___________.

Answer 14

heterogenous

Question 15

___________ receptors (red) constitutively cycle between endosomes and the plasma membrane.

Answer 15

Transferrin

Question 16

___________ receptors (green) are down-regulated by endocytosis followed by degradation in lysosomes.

Answer 16

Opioid

Question 17

Endocytosis of both transferrin and opioid receptors initiates in the same ___________- ___________ pits.

Answer 17

clathrin-coated

Question 18

___________ receptors are sorted to the recycling endosomes.

Answer 18

Transferrin

Question 19

___________ receptors are sorted to late endosomes/lysosomes.

Answer 19

Opioid

Question 20

___________ transporters are stored in recycling endosomes for fast mobilization.

Answer 20

Glut4

Question 21

AP2 binds to cargo receptors in ___________-rich membrane, then recruits

Answer 21

PI(4,5)P2

Question 22

Clathrin provides support for the best-studied route of ___________.

Answer 22

endocytosis

Question 23

The steps in endocytosis include marking the site, ___________ clathrin, bending the membrane, scission, and uncoating.

Answer 23

recruiting

Question 24

Molecules involved in marking the site for endocytosis include ___________.

Answer 24

Phosphatidylinositols (PIs)

Question 25

Molecules involved in recruiting clathrin include ____________________.

Answer 25

AP2, PI, cargo, AP180, Epsin

Question 26

Molecules involved in bending the membrane include ___________ domain proteins and ___________.

Answer 26

BAR, Epsin

Question 27

The molecule involved in scission is ___________.

Answer 27

Dynamin

Question 28

Molecules involved in uncoating include ___________, ___________, and ___________.

Answer 28

Hsc70, Auxilin, Synaptojanin

Question 29

AP180 recruits clathrin through ‘___________ ___________’ interactions.

Answer 29

fishing line

Question 30

The golgi is the sorting station for the ___________ pathway, while the endosome is the sorting station for the __________ pathway.

Answer 30

exocytic, endocytic

Question 31

___________ recognizes membrane regions rich in PI(4,5)P2, inserts into the membrane, bending it, and then recruits AP2 and clathrin.

Answer 31

Epsin

Question 32

Dynamin, a _____ase, pinches off the clathrin-coated vesicle (CCV) from the plasma membrane.

Answer 32

GTP

Question 33

True or False: COPI vesicles go from ER to Golgi, while COPII vesicles go from Golgi to ER.

Answer 33

False; COPI - Golgi to ER, COPII - ER to Golgi

Question 34

_______-microscopy can be used to measure and observe membrane binding.

Answer 34

TIRF