Vaginal hormonal cytology; Modified papanicolaou staining

Question 1

TRUE OR FALSE. Vaginal smears prepared for Vaginal hormonal cytology are best taken from the upper lateral third of the vaginal wall, because it is more accessible and less likely to be contaminated by cellular debris or vaginal discharges.

Answer 1

True

Question 2

TRUE OR FALSE. Smears should be examined first under low magnification to assess the quality of smear and staining, to detect the presence of RBC and leukocytes and the type of exfoliated cells, and to give a rough assessment of the proportion of mature superficial pyknotic acidophilic cells. A quantitative evaluation of the smear under 40 x objective is then in order.

Answer 2

True

Question 3

This test is not commonly used in today’s practice of gynecology.

Answer 3

Vaginal hormonal cytology

Question 4

Provides optimum nuclear detail information, which is important for cytologic evaluation of Pap smears, nongynecological and FNA cytologic specimens.

Answer 4

Modified papanicolaou staining

Question 5

TRUE OR FALSE. For Pap smears prepared with liquid- based technique, an automated stainer should be used.

Answer 5

True

Question 6

In Modified papanicolaou staining, to avoid contamination:

Answer 6

a. Stain non-gynecologic and FNA specimens separately.
b. Do not stain fluid specimens in close proximity to other cases.
c. Stain suspicious cases separately when necessary.
d. Assess cellularity of fluids and FNA specimens and stain those with high potential for cross contamination separately.