Staining method in cytology Flashcards
It is the routine staining procedure used in cytopathology laboratory and is a polychrome staining reaction that results in well stained nuclear chromatin, differential cytoplasmic counterstaining and cytoplasmic transparency.
Papanicolaou staining method
It is considered to be the staining method of choice for exfoliative cytology.
Papanicolaou staining method
the gold standard staining method in cytology.
Pap stain
What are the 2 Cytoplasmic stains?
OG-6 and EA-36.
It is is a monochrome stain.
OG-6
It is a polychrome stain.
EA-36
Formalin-fixed cytology preparations must be stained with either _______ or ______.
H&E or Papanicolaou stain.
Stains used for cytological diagnosis of non –gynecological specimens and Dip Quick for rapid staining.
May-Grunwald- Giemsa (MGG) and modified Wright-Giemsa stains
It is an aqueous stains as opposed to the methyl-alcohol based stains used in hematology.
Romanowsky stains
It is generally used for air-dried fine needle aspirates.
Romanowsky stains
Most frequently used stains for cytochemistry:
for glycogen
for hemosiderin
for mucins
for fungal organisms
for acid fast bacilli
Periodic Acid Schiff (PAS) for glycogen
Perl’s stain for hemosiderin
Alcian Blue for mucins
Grocott Methenamine Silver (GMS) for fungal organisms and
Ziehl-Neelsen stain for acid fast bacilli
TRUE OR FALSE. The use of coated slides will prevent washing off of the cells during processing and staining. Both peroxidase and alkaline phosphatase may be used for immunohistochemistry.
True
