Immunohistochemistry

Question 1

Studies for cell surface antigens on cell smears, imprints, and cytocentrifuge preparations show sensitivity equal to, or greater than, that of histologic sections.

Answer 1

Immunohistochemistry

Question 2

TRUE OR FALSE. False negative immunoperoxidase results for intracellular antigens are more common in histologic specimens than cytologic samples.

Answer 2

False. False negative immunoperoxidase results for intracellular antigens are more common in cytologic samples than in histologic specimens.

Question 3

TRUE OR FALSE. Cell suspensions are routinely used for routine immunohistochemistry.

Answer 3

False. Cell suspensions are seldom used for routine immunohistochemistry

Question 4

TRUE OR FALSE.Samples stored for several months, either at room temperature, 4degC or -20degC can be processed for DNA and protein analysis.

Answer 4

True

Question 5

TRUE OR FALSE.Polymerase chain reaction (PCR) and subsequent direct sequencing can be used to analyze mutations of p53 and other genes from relatively few suspicious or malignant cells.

Answer 5

True

Question 6

It is the most objective technique for assessment of Her2/neu of fine needle aspirates for assessment of breast cancer.

Answer 6

Fluorescence In Situ Hybridization (FISH)