Body cavity effusion; Fixation

Question 1

TRUE OR FALSE. Fluid specimens should be collected in a clean, non-sterile dry container, and submitted fresh to the laboratory.

Answer 1

True

Question 2

If transportation is delayed, the specimen should be placed in the ________.

Answer 2

refrigerator

Question 3

TRUE OR FALSE. Use of formalin, alcohol or any other preservative should be avoided.

Answer 3

True

Question 4

TRUE OR FALSE.Heparin interferes with cytologic preparation and evaluation.

Answer 4

false. It does not interfere

Question 5

The addition of what type of solution is recommended to be added to the cerebrospinal fluid (CSF) is if a delay in processing is anticipated.

Answer 5

ethyl alcohol

Question 6

For CSF specimens, a minimum amount of _____ is necessary for cytologic evaluation.

Answer 6

1 cc.

Question 7

TRUE OR FALSE. Exfoliated cells decompose rapidly, and drying rapidly destroys cellular and nuclear details, resulting in a specimen that is inadequate for diagnosis.

Answer 7

True

Question 8

What should be done to prevent the destruction of cellular and nuclear details in exfoliative cells.

Answer 8

spray fixative or aqueous- alcoholic solutions containing polyethylene glycol or Carbowax.

Question 9

If smears cannot be made immediately, the collected material should be placed in _____ for all types of effusions.

Answer 9

50% alcohol

Question 10

Saccomano preservative is composed of _____ and _____.

Answer 10

50% alcohol and carbowax

Question 11

TRUE OR FALSE. Minimum fixation time varies. Some types of smears can be fixed in 10 minutes (with 90% alcohol). A longer fixation is usually not needed.

Answer 11

True

Question 12

Process of submerging of freshly prepared smears immediately in a liquid fixative is called ____

Answer 12

wet fixation

Question 13

This is the ideal method for fixing all gynecological and non- gynecological smears.

Answer 13

wet fixation

Question 14

This is the ideal fixative recommended in most laboratories for cytological specimen.

Answer 14

95% Ethyl Alcohol (Ethanol)

Question 15

As a dehydrating agent, it causes enough cell shrinkage to yield optimal chromatin detail characteristics of cytological preparations.

Answer 15

95% Ethyl Alcohol (Ethanol)

Question 16

This fixative was originally recommended by Papanicolaou.

Answer 16

Ether alcohol mixture

Question 17

It consists of equal parts of ether and 95% ethyl alcohol. It is an excellent fixative, but ether is not used in most of the laboratories because of its safety hazards.

Answer 17

Ether alcohol mixture

Question 18

It is an acceptable substitute for 95% ethanol.

Answer 18

100% Methanol

Question 19

It causes less shrinkage and is more expensive than ethanol.

Answer 19

100% Methanol

Question 20

These are substitutes for wet fixatives.

Answer 20

Carbowax (Polyethylene Glycol) fixatives

Question 21

They are either aerosols applied by spraying the cellular samples or a liquid base, which is dropped onto the slide.

Answer 21

Carbowax (Polyethylene Glycol) fixatives

Question 22

It is a special purpose fixative used to hemolyze the red blood cells in hemorrhagic samples.

Answer 22

Carnoy’s fixative

Question 23

It is an excellent nuclear fixative as well as preservative for glycogen but results in considerable shrinkage of cells and tends to produce over staining in hematoxylin.

Answer 23

Carnoy’s fixative