Body cavity effusion; Fixation Flashcards
TRUE OR FALSE. Fluid specimens should be collected in a clean, non-sterile dry container, and submitted fresh to the laboratory.
True
If transportation is delayed, the specimen should be placed in the ________.
refrigerator
TRUE OR FALSE. Use of formalin, alcohol or any other preservative should be avoided.
True
TRUE OR FALSE.Heparin interferes with cytologic preparation and evaluation.
false. It does not interfere
The addition of what type of solution is recommended to be added to the cerebrospinal fluid (CSF) is if a delay in processing is anticipated.
ethyl alcohol
For CSF specimens, a minimum amount of _____ is necessary for cytologic evaluation.
1 cc.
TRUE OR FALSE. Exfoliated cells decompose rapidly, and drying rapidly destroys cellular and nuclear details, resulting in a specimen that is inadequate for diagnosis.
True
What should be done to prevent the destruction of cellular and nuclear details in exfoliative cells.
spray fixative or aqueous- alcoholic solutions containing polyethylene glycol or Carbowax.
If smears cannot be made immediately, the collected material should be placed in _____ for all types of effusions.
50% alcohol
Saccomano preservative is composed of _____ and _____.
50% alcohol and carbowax
TRUE OR FALSE. Minimum fixation time varies. Some types of smears can be fixed in 10 minutes (with 90% alcohol). A longer fixation is usually not needed.
True
Process of submerging of freshly prepared smears immediately in a liquid fixative is called ____
wet fixation
This is the ideal method for fixing all gynecological and non- gynecological smears.
wet fixation
This is the ideal fixative recommended in most laboratories for cytological specimen.
95% Ethyl Alcohol (Ethanol)
As a dehydrating agent, it causes enough cell shrinkage to yield optimal chromatin detail characteristics of cytological preparations.
95% Ethyl Alcohol (Ethanol)
This fixative was originally recommended by Papanicolaou.
Ether alcohol mixture
It consists of equal parts of ether and 95% ethyl alcohol. It is an excellent fixative, but ether is not used in most of the laboratories because of its safety hazards.
Ether alcohol mixture
It is an acceptable substitute for 95% ethanol.
100% Methanol
It causes less shrinkage and is more expensive than ethanol.
100% Methanol
These are substitutes for wet fixatives.
Carbowax (Polyethylene Glycol) fixatives
They are either aerosols applied by spraying the cellular samples or a liquid base, which is dropped onto the slide.
Carbowax (Polyethylene Glycol) fixatives
It is a special purpose fixative used to hemolyze the red blood cells in hemorrhagic samples.
Carnoy’s fixative
It is an excellent nuclear fixative as well as preservative for glycogen but results in considerable shrinkage of cells and tends to produce over staining in hematoxylin.
Carnoy’s fixative
