Papanicolaou stain Flashcards
Multichromatic staining cytological technique that is used to differentiate cells in smear preparations of various bodily secretions, including gynecological smears (Pap smears), sputum, brushings, washings, urine, cerebrospinal fluid, abdominal fluid, pleural fluid, synovial fluid, seminal fluid, fine needle aspiration material, tumor touch samples, or other materials containing cells.
Papanicolaou stain
It is used to stain the nuclei.
Hematoxylin (nuclear stain)
It stains keratin and was originally used to stain the small cells of keratinizing squamous cell carcinoma present in sputum.
Orange G
stains the superficial epithelial squamous cells, nucleoli, cilia, and red blood cells.
Eosin Y
stains the cytoplasm of other cells, including non- keratinized squamous cells.
Light Green SF yellowish
Since Light Green SF yellowish is quite expensive and difficult to obtain, what can be used as an alternative stain?
Fast Green FCF
Stains nothing and in contemporary formulations it is often omitted.
Bismarck brown Y
When papanicolaou is performed properly, the stained specimen should display hues from the entire spectrum. What are the colors involved?
red, orange, yellow, green, blue, and violet.
Color of Cell nuclei.
crisp blue to black.
Color of cells with high content of keratin and glycogen.
yellow
Color of Superficial cells.
orange to pink
Color of intermediate and parabasalcells.
turquoise green to blue
What type of cell often stain both green and pink at once.
Metaplastic cells
TRUE OR FALSE. OG and EA stains lose strength more rapidly than hematoxylin and should be replaced every week or as soon as the staining of cells are no longer sharp and crisp.
True
TRUE OR FALSE. Wet fixation is time consuming, shows drying artifacts and less retention of material compared to air dried smears.
True
