Slide preparation Flashcards
TRUE OR FALSE.When a solid lesion is aspirated, usually a few drops from the tip of the needle has the most diagnostic material for cytologic evaluation.
True
TRUE OR FALSE. On the other hand when the specimen is bloody, the diagnostic cells are diluted and hard to find on a direct smear.
True
recommended preparation of maximum _______: using ______drops on each slide and using _______.
4 slides; 1-2; slide-pull technique
In slide prep, what type of preservative solution is used to rinse the needle.
Saccomano fluid.
What type of consistency does an ideal aspirate has?
creamy consistency with numerous cells suspended in a small amount of tissue fluid
Colloid, mucin and smears are stained with _______.
hematological stains (May- Grunwald-Giemsa)
Rapid stains like ______ are useful in preliminary assessment of adequacy of the sample.
Diff Quik (2-3 mins)
Smears to be stained by Papanicolaou (Pap) or hematoxylin and eosin (H&E) should be rapidly fixed in alcohol (wet fixation) to _________ and _________.
show nuclear details; allow better identification of malignant cell
For _______ specimens, a drop or two should be released onto a slide, smeared by two-slide pull method and fixed immediately.
Transbronchial Fine needle Aspiration (FNA)