May-Grunwald giemsa stain Flashcards
One of the common Romanowsky stains used in cytology.
May-Grunwald giemsa stain
It is useful for studying cell morphology in air-dried smears.
May-Grunwald giemsa stain
It is superior to Papanicolaou stain when studying the cytoplasm, granules, vacuoles, and basement membrane material.
May-Grunwald giemsa stain
TRUE OR FALSE. For nuclear staining Papanicolaou stain is superior than May-Grunwald giemsa stain.
True
Improper washing properly leads to the formation of ___.
dye artifacts.
TRUE OR FALSE. Staining result is dependent on pH. Alkaline pH increases blue and acidic pH pink or reddish tinge in the stained specimen.
True
TRUE OR FALSE. Under-stained smears can be re- stained for further periods to increase staining intensity.
True
TRUE OR FALSE. Staining solutions are not required to be filtered daily in order to remove any loose cells/cell components.
False. Staining solutions should be filtered daily in order to remove any loose cells/cell components.
TRUE OR FALSE. When precipitates are formed on the slide, the stains should be mixed thoroughly or replaced.
True
TRUE OR FALSE. It is important to renew alcohol baths (ethanol 96% and 100%) regularly in order to achieve good differentiation and stain transparency.
True
TRUE OR FALSE. The last alcohol, xylene or xylene-substitute baths must be absolutely clean and free of water.
True
Any water remaining can cause the slide to become ____ as a result of oxidation.
decolored
TRUE OR FALSE. Rehydration of air-dried smear with normal saline will restore the transparency of the cell or hemolyzed RBCs, while the use of 4% formaldehyde/65% ethanol fixative can reduce the time needed for proper fixation and staining of the smears.
True
