Urea Broth/ Urease Test Flashcards
1
Q
test used to determine urease enzyme
A
urea broth/ urease test
2
Q
presence of this enzyme breaks down urea into CO2 (carbon dioxide) and NH3 (ammonia);
A
urease
3
Q
waste product of amino acid protein breakdown
A
urea
4
Q
Urea broth contains both _______ & __________. What color is the broth?
A
urea and pH indicator phenol red; broth is yellow in color
5
Q
Is ammonia acidic or basic? Will it lower or raise pH?
A
Basic/Alkaline; raise pH
6
Q
Urease Positive test has a color change of yellow to ______? Why
A
Positive= Pink/red ; bc NH3 is alkaline and will raise pH
7
Q
A broth that is negative for urease will be _____
A
yellow/orange in color
