Microscope/ Wet Mount Techniques

Question 1

Purpose of Microscope

Answer 1

create magnified image of objects to small to be seen with the eye only

Question 2

What does Brightfield microscope use to view specimens?

Answer 2

Light and Glass

Question 3

Purpose of Oil Immersion

Answer 3

prevents refraction; reduces light scatter and increases resolution; directs more light to objective lens

Question 4

What objective is immersion oil used with?

Answer 4

Can only be used with 100x objective

Question 5

Scanning Objective

Answer 5

4x

Question 6

Low Power Objective

Answer 6

10x

Question 7

High Power Objective

Answer 7

40x

Question 8

Oil Immersion

Answer 8

100x

Question 9

Parfocal means

Answer 9

when one objective lens is in focus, the other objective lens will also be in focus

Question 10

Lens closest to the eye, with magnification of 10x

Answer 10

Ocular Lens

Question 11

Lens closest to specimen

Answer 11

Objective Lens

Question 12

Focuses light on specimen

Answer 12

Condenser lens

Question 13

moves stage back and forth or left and right

Answer 13

stage control

Question 14

moves stage up and down

Answer 14

focus control

Question 15

used under low power

Answer 15

coarse focus

Question 16

used under high power

Answer 16

fine focus

Question 17

Magnification is the

Answer 17

increase in image size

Question 18

Resolution is the

Answer 18

ability to distinguish fine detail and see clearly

Question 19

Contrast is the

Answer 19

ability to distinguish objects from their background

Question 20

Wet Mount Technique allows you to

Answer 20

view living organisms, see specimen behavior and motile organisms

Question 21

How do you create a wet mount?

Answer 21

Using pipette, drop liquid in center over slide, use cover slip and avoid air bubbles

Question 22

Special Type of Wet Mount technique where a depression slide is used, and drop of medium is covered by a cover slip

Answer 22

Hang drop technique

Question 23

What are the purposes of stains?

Answer 23

Stains are used to see cell structures by enhancing contrast

Question 24

Directional/ purposeful motility is a

Answer 24

positive test/ true motility, as most motile organisms change position w/ respect to one another (compared to Brownian movement)

Question 25

What is Brownian Movement?

Answer 25

Molecules in liquid striking objects causing random shaking or bouncing where organisms move at same rate and remain in same relative positions

Question 26

study of tissue

Answer 26

Histology

Question 27

study of cells

Answer 27

cytology

Question 28

study of disease

Answer 28

Pathology

Question 29

What are the cons of staining cells?

Answer 29

Stains can be toxic and are used on dead cells so movement cannot be seen