Tryptone Broth/Indole Test

Question 1

Purpose of indole test?

Answer 1

to see if bacteria has the ability to degrade amino acid tryptophan and produce indole

Question 2

This broth/test helps with the determination of what type of bacteria?

Answer 2

gram negative bacilli

Question 3

If tryptophanase is present, the tryptophan will be broken into ____, ____ and ______?

Answer 3

Ammonia, pyruvate and indole

Question 4

_______ is added will react with indole if present, and results in a ____ ring at top of test tube?

Answer 4

Kovac’s Reagent ; cherry red