Special Stains- Flagella Stain

Question 1

Purpose of Flagella stain

Answer 1

To observe flagella since it is usually too thin to be seen with microscope. Stain makes flagella visible under microscope

Question 2

Flagella Stain and Technique

Answer 2

Prepare smear, Add Flagella Mordant for 10 mins, Rinse w/ distilled water, Add carbolfuchsin for 5 mins, Rinse w/ distilled water, let air dry