Unusual Bacteria Flashcards
What do the chlamydiaceae family have in common about intracellular and extracellular living?
- cannot make ATP
- elementary body (extracellular) ▶️ infective form, inactive
- reticular body (intracellular) ▶️ active, replicating
Serotypes of chlamydia trachomatis involved in sexually transmitted diseases (non-gonococcal urethritis, cervicitis, PID) and inclusion conjunctivitis and neonatal pneumonia?
Serotypes D-K
Serotypes involved in Lymphogranuloma venereum caused by chlamydia trachomatis?
Serotypes L1, L2, L3
*L=Lymphogranuloma
Serotypes involved in trachoma caused by chlamydia trachomatis
Serotypes A, B, Ba, C
How do you diagnose chlamydia trachomatis infection?
PCR, DNA probes
Stain smear or scraping ▶️ giemsa, iodine, fluorescent antibody ▶️ cytoplasmic inclusions
Serodiagnosis ▶️ DFA, ELISA
Causes of walking pneumonia
Chlamydophila pneumoniae
Mycoplasma pneumoniae
Psittacosis (Parrot fever), clinical presentation and microorganism that caused it.
Atypical pneumonia + hepatitis
Chlamydophila psittaci
How is the rash in Rocky Mountain spotted fever? Pathogenesis.
Petechial rash initiates on ankles and wrist ▶️ trunk, palms, soles, face (centripetal rash)
Invading endothelial cells of capillaries ▶️ vasculitis
Diagnosis of Rocky Mountain spotted fever
Serological IFA ▶️ ⬆️ fourfold titer
Weil-Felix test (+)
Clinical presentation of ehrlichiosis, agents that cause it and difference in mechanism of infection between them?
Similar to Rocky Mountain spotted fever BUT, NO RASH
leukopenia
Thrombocytopenia
- E. Chaffeensis ▶️ monocytes and macrophages infection
- Anaplasma phagocytophila ▶️ neutrophils infection
Diagnose of ehrlichiosis
Giemsa-stained blood film ▶️ morulae: mulberry-like structures inside infected cells
Serology
DNA probe
Common confection in Lyme disease. When do you suspect it? Why does it occur?
Borrelia burgdorferi + Anaplasma phagocytophila
Very high fever than Borrelia alone
Have same geographic range as Lyme disease and same vector (Ixodes ticks)
Virulence factors associated to mycoplasma pneumoniae
P1 protein ▶️ attach respiratory epithelium
(-) ciliary action
Produce reactive oxygen species (ROS) and cytolytic enzymes ▶️ necrosis respiratory epithelium
Superantigen
Diagnose of walking pneumonia by mycoplasma pneumoniae
ELISA, immunofluorescence sensitive, specific
Culture: Mulberry-shaped colonies on sterol-containing media
Positive cold agglutinins (+)
