Unit 8 Quiz #2 Flashcards
Gel electrophoresis
seperates DNA based on size by applying electric field
gel filter
filter that sorts dna strands
electropherosis
pushes dna by adding electrical current
what is needed to analyze electrophoresis results
dye or stail like ithedium bormide
buffer
salt water solution that lets electrical charge flow though gel
comb
creates well in gell for dna
positive/negative control
positive means experiment is good, negative means experiment is bad
2 functions of loading buffer
1) increases density
2) provides marker/dye
dna size standard
contains dna strands of known length to give you reference by which to estimate length of DNA stand in your sample
if bubbles are formed what does that mean
electricity is flowing and electrophoresis is occuring
what does pcr stand for
polymerase chain reaction
use of pcr
dna polymerase
primer
short pieces of DNA attached on either end of target
dna polymerase
enzyme that copies dna
why are 2 primers needed on each side
dna polymerase can’t stop copying a segment of DNA already established to add on to it
what happens when temp is 90
dna stands come apart
called denaturation
what happens at 50-60
primers bind to single strands of DNA
called annealing
what happens at 72
taq polymerase adds nucleotides to primers and builds new stands of dna
called extension
