Unit 6a - The Molecular Basis of Inheritance Flashcards
Chargaff’s Rules
dna composition varies between species
Base pair ratios
a% = t% + c% = g%
Purines
double ring; A + G , paired with pyrimidine
Pyrimidine
single ring: T + C, paired with purine
Hydrogen bond
between base pairs of the two strands that hold the molecule together
The bond between A + T
double bond
The bond between C + G
triple bond
Prokaryotic DNA
double stranded
circular
one chromosome
in cytoplasm
no histones
super-coiled DNA
Eukaryotic DNA
double stranded
linear
1+ chromosome (usually)
DNA wrapped around histones
forms chromatin
Antiparallel
one strand 5’ to 3’ , the other is 3’ to 5’
Define Semi-conservative
half of the DNA is original ; half is newly synthesized
Function of Helicase
unzip and unwind DNA
Function of Polymerase
adds nucleotide to the new DNA strands
Function of Primase
adds RNA primer
RNA primer
provides a binding site for the DNA polymerase