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HEMA 1 LEC > Unit 3.2 BLOOD CELL COUNT > Flashcards

Unit 3.2 BLOOD CELL COUNT Flashcards

1
Q

Numerical evaluation of formed elements of blood Estimation of the number of blood cells in a known volume of blood.

A

HEMOCYTOMETRY

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2
Q

Units used in reporting cell counts
Relative Count:
Absolute Count:

A

%
cells X 10^12/L; cells X 10^9/L; cells /uL

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3
Q

Diluting pipets:

A

Automatic

Non automatic

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4
Q

microglass capillary pipets that automatically suck in just the right amount of sample;

A

Automatic pipets

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5
Q

connected to a plastic container containing just the right amount of sample (Eg. Trenner and Unopette)

A

Automatic pipets

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6
Q

Non-automatic pipets

A

Thoma pipets (2BC and RBC diluting pipets)

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7
Q

Red

A

RBC pipet

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8
Q

White

A

WBC pipet

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9
Q

Larger bulb

A

RBC pipet

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10
Q

Smaller bulb

A

WBC pipet

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11
Q

RBC pipet vol in bulb

A

100

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12
Q

WBC pipet vol in bulb

A

10

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13
Q

Smaller bore

A

RBC pipet

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14
Q

Larger bore

A

WBC pipet

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15
Q

RBC pipet calibration

A

.5; 1; 101

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16
Q

WBC pipet calibration

A

.5; 1; 11

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17
Q

RBC pipet usual dilution

A

1:200

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18
Q

WBC pipet usual dilution

A

1:20

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19
Q

RBC pipet dilution range

A

1:100-1:1000

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20
Q

WBC pipet dilution range

A

1:10-1:100

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20
Q

WBC pipet dilution range

A

1:10-1:100

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21
Q

1:10
WBC

A

0.1 - 3.0

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22
Q

1:20
WBC

A

3.1 - 30.0

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23
Q

1:100
RBC

A

> 30?0

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24
Q

1:200
RBC

A

/= 100.0

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25
Q

A counting chamber designed for counting cells.

A

Hemocytometer

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26
Q

It consists of a thick microscope glass with a rectangular indentation that creates a chamber.

A

Hemocytometer

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27
Q

This chamber has grid lines so that the area and the depth are known.

A

Hemocytometer

28
Q

By observing a (?), it is possible to count the number of cells in a specific volume of fluid, and thereby calculate the concentration of cells in the fluid overall volume.

A

defined area of the grid

29
Q

Types of Hemocytometer:
A. According to type:
B. According to Rulings:

A
  1. Open type (Spencer, Levy, Levy-Hauser)
  2. Closed type (Thoma-Zeis)
  3. Addis; Eton; Petroff
  4. Thoma; Tuerk; Bass Jones
  5. Improved Neubauer
  6. Fuchs-Rosenthal
30
Q

0.2 mm

A

Fuchs-Rosenthal
Speirs-Levy

31
Q

2 ruled areas; 4mm x 4mm each

A

Fuchs-Rosenthal

32
Q

Each ruled area is divided into 16 large squares

A

Fuchs-Rosenthal

33
Q

Each large square is further divided into 16 small square

A

Fuchs-Rosenthal

34
Q

3.2 mm3/sq

A

Fuchs-Rosenthal

35
Q

Total= 6.4 mm3

A

Fuchs-Rosenthal

36
Q

4 ruled areas; 2mm x 5mm each

A

Speirs-Levy

37
Q

Each ruled area is divided into 10 equal squares arranged in 2 rows

A

Speirs-Levy

38
Q

2.0 mm3/sq

A

Speirs-Levy

39
Q

Total= 8 mm3

A

Speirs-Levy

40
Q

0.1 mm

41
Q

2 ruled areas; 3mm x 3mm each

42
Q

Each side is divided into 9 equal squares

43
Q

0.9 mm3/sq

44
Q

Total= 1.8 mm3

45
Q

for WBCs

46
Q

􏰁 for RBCs & platelets

47
Q

Locate the first square. Start counting going towards the[?], then down towards left until the entire square is covered.

48
Q

Count all cells inside the small and large squares. INCLUDE the cells touching the[?] boundary lines, EXCLUDE the cells touching the [?]boundary lines.

A

LEFT & TOP
BOTTOM and RIGHT

49
Q

Diluting fluids: Ideal Characteristics

􏰁 (?) & economical; Easy to secure and prepare and stable.
􏰁 With(?)
􏰁 With(?)
􏰁 With
􏰁 Non-(?)and non-(?)
􏰁(?) for RBCs; (?) for WBCs

A

Cheap

preservative action

high specific gravity

buffer action

allergenic; corrosive

Isotonic; Hypotonic

50
Q

WBC diluting fluids:

A
  1. 2-3% Gl Aceticacid
  2. 1%HCl
    3.Turk’s solution - glacial acetic acid (3mL); DH2O(96mL)
    + 1% aqueous Gentian violet (1 mL)
51
Q

RBC diluting fluids

A
  1. Hayem’s
  2. Gower’s
  3. Toisson’s
  4. Bethel
  5. Formol-Citrate (Dacie’s fluid)
  6. NSS
  7. 3.8% Sodium Citrate
52
Q

Computation: Formula for manual cell count
Total Cell count/cumm =
Dilution factor (DF) =
Corrected WC =

53
Q

Reporting:

54
Q

Reference values:
RBC count:
WBC count:
Platelet count:

A

(Male)
4.5 -5.9 x 1012/L
(Female)
4.1 -5.1 x 1012/L

4.4 -11.3 x 109/L

150 -450 x 109/L

55
Q

Errors in Manual Hemocytometry:

A
  1. Technical/Human Errors (Errors in the dilution, loading of chamber, identification of cells, etc.)
  2. Equipment/Standard Errors (Calibration errors of Thoma pipets)
  3. Inherent/Field Error (Distribution of cells)
56
Q

Quality Control in Manual Cell Counting
􏰆 Check specimens for[?]
􏰆 Only[?] should be used
􏰊 There should be NO MORE THAN a [?]between the highest and lowest number of WBCs
found among the large squares counted; NO MORE THAN[?] for the RBC count.

A

clot
NBS Thoma pipets and hemocytometers
15-cell difference; 20 cells

57
Q

Initiates mold formation & rouleaux formation

58
Q

Can stand for a long time and no corrosive effect

59
Q

Prevents rouleaux formation & precipitation or CHONS

60
Q

Initiates mold formation (should be filtered before use)

A

Toisson’s

61
Q

Has high specific gravity and with stain

A

Toisson’s

62
Q

Formol-Citrate (Dace fluid) -

A

10ml 50% formalin; 990ml 3% Na citrate,

63
Q

BEST RBC diluting fluid

A

Formol-Citrate (Dace fluid)

64
Q

Has preservative action & prevents mold formation

A

Formol-Citrate (Dace fluid)

65
Q

Does not alter cell morphology

A

Formol-Citrate (Dace fluid)

66
Q

Used in cases of emergency when no other RBC diluting fluids are available

67
Q

Ideal to use in cases of excessive rouleaux and autoagglutination

HEMA 1 LEC (15 decks)

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