Unit 3-Molecular Genetics Lesson 1 Flashcards
Who found a phosphorous rich substance in the nucleus?
Friedrich Miescher
What was the substance found by Friedrich Miescher called?
Nuclein
What did Griffith’s experiment demonstrate?
Dead virulent bacterial cells transformed non-virulent cells.
What did Hammerling hypothesize about the nucleus?
The nucleus contains the hereditary information.
What was the main aim of Hershey and Chase’s experiment?
To rule out protein in favor of DNA as the hereditary material.
Which organisms did Hershey and Chase use in their experiments?
Bacteriophages (viruses that infect bacteria).
What happened in Hershey and Chase’s 1st experiment?
A virus with radioactively labelled DNA infected E. coli bacteria, revealing most of the radioactive DNA in the bacteria.
What was the key finding from the 1st experiment of Hershey and Chase?
The viral DNA had entered the bacteria.
What was the setup of Hershey and Chase’s 2nd experiment?
A virus with radioactively labelled protein infected E. coli bacteria, using the same separation procedure.
What did Hershey and Chase find in their 2nd experiment regarding the radioactively labelled protein?
Most of it was found in the liquid medium, not in the bacteria.
What conclusion did Hershey and Chase draw from their two experiments?
Viral DNA was transferred to bacterial cells, holding the genetic information needed for reproduction.
Who are the three key figures associated with the discovery of DNA structure?
Francis Crick, James Watson, and Rosalind Franklin.
What is Chargaff’s Rule?
Adenine must pair with Thymine, and Guanine must pair with Cytosine.
What does complementary base pairing mean?
If you know the sequence of one strand, you can find the sequence of the complementary strand.
What are the characteristics of the DNA strands?
They are antiparallel.
