Unit 2 - Cell Biology Flashcards
why do we use buffer solution in cell franctionatio
to keep the pH constant because if pH changes then enzymes in the cell could denature
why do we use cold temperatures in cell fractionation
- to slow down enzymic activity - prevent destructive enxymes from damaging organelles
what does cell fractionation mean
the seperation of all the different organelles
what is meant by the term resolution
the minimum distance between 2 objects where they can still be seen as 2 seperate objects
whats an advantage and a disadvantage to a light microscope
we can look at living cells but it has a poor resolution
how many micrometres is 1mm
1000
whats an advantage and disadvantage to electron microscopes
- they have better resolution than light microscopes
- you cannot view living specimens
whats an advantage and disadvantage to a TEM
- high resolution
- 2d image
whats an advantage and disadvantage to a SEM
- 3d image
- lower resolution
give 3 examples of eukaryotic organisms
animals
plants
fungi
give 2 examples of prokaryotic organisms
bacteria
archea
what are 3 key features to eurkaryotic cells
- they have their DNA in a membrane bound nucleus
- their DNA is tightly wrapped around histone proteins
- membrane bound organelles are present
what is the role of ribosomes
site of protein synthesis
name 2 polysaccharides which make up cell walls in plants and fungi
cellulose for plant
chitin for fungal
what are 3 major differences between prokaryotic and eukaryotic cells
- prokaryotic cells are much smaller
- DNA is free flowing in cytoplasm in prokaryotic cells
- ribosomes in prokaryotic cells are smaller than in eurkayotic