Unit 2 Flashcards
4 fundamental components of a cell
- cell membrane / plasma
- chromosomes (nucleic acids)
- ribosomes
- cytoplasm
what does the nucleus do?
stores genetic information
plasma membrane?
consists of a phospholipid bilayer and may contain cholesterol in humans
ribosomes?
used to make proteins
mitochondria?
used in the formation of ATP
lysosome?
contains digestive enzymes, and plays a role in phagocytosis
Replication of DNA enzyme:
DNA polymerase
Transcription - mRNA synthesized using DNA as a template, enzyme:
DNA-dependent RNA polymerase
translation - proteins are synthesized using mRNA as template, requires:
ribosomes
size comparison b/w euk & pro
euk - larger
pro - smaller
DNA - genome location
euk - nucleus & DNA packaged using histones
pro - nucleoid (not surrounded by membrane)
DNA - # of chromosomes
euk - multiple
pro - one
DNA - # of copies of each gene
euk - diploid
pro - haploid
DNA - shape of chromosomes
euk - linear
pro - circular
DNA - plasmids = extrachromosomal DNA that does not carry essential genes
euk - absent
pro - present
Cell division
euk - mitosis
pro - binary fission
genetic variation
euk - meiosis
pro - horizontal gene transfer
types of horizontal gene transfer:
- transformation
- transduction
- conjugation
- take up DNA from environment
- bacteriophage (virus attacks bacteria) transfers DNA from one bacterium to another
- one bacterium directly transfers DNA to another using a pilus
membrane-bound organelles
euk - present
pro - absent
ribosomes e / p
euk - free and bound
pro - free (smaller & less dense than euk)
plasma membrane: structure & presence of sterols
euk - phospholipid bilayer & present
pro - phospholipid bilayer & absent
cell wall - component
euk: protozoans & helminths: none algae: silica or cellulose fungi: chitin pro: archaea: pseudopeptidoglycan bacteria: peptidoglycan
describe structure of peptidoglycan
multiple polysaccharides of alternating G & M, cross-linked with peptide chains
gram positive
thick peptidoglycan layer
teichoic acids embedded, used to stabilize layer
no outer membrane
no periplasm
gram negative
thin peptidoglycan layer no teichoic acids contains outer membrane outside of layer lipopolysaccharide & lipoproteins periplasm - gel material b/w outer membrane & peptidoglycan
glycocalyx - extracellular later
euk - present
pro - present:
capsule - rigid and organized
slime layer - loosely attached & less organized
positive phototaxis
toward stimulus (light)
negative phototaxis
away stimulus (light)
structures used for movement
euk: protozoa: psuedopods, cilia, flagella algae: flagella fungi & helminths: no movement pro: flagella
flagella movement in aqueous solution
euk: undulating
pro: rotating
atrichous
no flagella
monotrichous
one flagella
lophotrichous
tuff flagella
amphitrichous
flagella at both ends
peritrichous
flagella all over surface
run: all flagella are rotating counterclockwise
tumble: all flagella are rotating clockwise
structures only found in prokayotes
inclusions: storages of excess nutrients (gas vesicles used to control buoyance)
fimbriae: short bristle-like appendages and used for attachment
pilus: long protein appendages used for DNA transfer
location of metabolism in eukaryotes
cytoplasm, mitochondria
location of metabolism in prokaryotes
cytoplasm
aerobic cellular respiration in prokaryotes
glucose -> glycolysis/E-D pathway -> acetyl CoA formation (krebs cycle) -> regeneration of NAD+ using ETC -> ATP formation
anaerobic cellular respiration inorganic molecules
nitrate + electrons = nitrous oxide or nitrogen gas
sulfate + electrons = hydrogen sulfide
carbonate + electrons = methane
fermentation
glucose -> glycolysis -> regeneration of NAD+ using fermentation pathways -> ATP formation
fermentation: O2 & ETC not present, regeneration of NAD+ is:
- lactic acid: pyruvate + NADH -> lactate + NAD+ = pyruvate final electron acceptor
- ethanol fermentation:
a. pyruvate -> CO2 + acetaldehyde
b. acetaldehyde + NADH = ethanol + NAD+
acetaldehyde is final electron acceptor
lipid catabolism
secrete extracellular lipases -> breakdown triglycerides to fatty acids -> B-oxidation to form acetyl-coa -> enter krebs
protein catabolism
secrete extracellular proteases -> break down protein to amino acids -> deaminases used to remove amino group from amino acid -> releases ammonium -> remaining carbon chain is metabolized
photo =
capable of photosynthesis for ATP formation
chemo =
relies on chemicals for ATP formation
auto =
can use both organic (glucose) & inorganic carbon (CO2)
hetero =
can only use organic carbon