2401 unit 5 Flashcards

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1
Q

optimum growth temperature

A

the temp where growth rates are the highest

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2
Q

minimum growth temperature

A

the lowest temp in which the organism can still grow and replicate

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3
Q

max growth temp

A

the highest temp in which the organism can still grow and replicate

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4
Q

temp for psychrophiles

A

below 0 to 15

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5
Q

temp for psychrotrophs

A

4 - 25

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6
Q

temp for mesophiles

A

20 - 45

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7
Q

temp for thermophiles

A

50 - 80

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8
Q

temp for hyperthermophiles

A

80 - 100

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9
Q

optimal pH for acidophile

A

less than 5.5

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10
Q

optimal pH for neutrophile

A

~ 7

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11
Q

optimal pH for alkaliphile

A

8 - 11.5

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12
Q

hypotonic environments

A

lower concentration of solutes in the environment than the cell

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13
Q

hypertonic environments

A

higher concentration of solutes in the environment than the cell

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14
Q

for hypotonic, the movement of water into cell leads to

A

lysis

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15
Q

for hypertonic, the movement of water out of the cell leads to

A

crenation

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16
Q

microbes with cell wall are susceptible only to

A

hypertonic

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17
Q

hypotonic for cell wall microbes

A

water still moves into the cell; presence of the cell wall prevents lysis

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18
Q

hypertonic for cell wall microbes

A

movement of water out of the cell eventually leads to plasmolysis (separation of the plasma membrane from the cell wall)

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19
Q

halophiles

A

organisms that require salt for growth

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20
Q

halotolerants

A

organisms that do not need salt for growth but can survive and grow in the presence of high salt environments

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21
Q

macronutrients are

A

elements needed in large amounts = carbon, hydrogen, oxygen, phosphorus, sulfur and nitrogen

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22
Q

micronutrients are

A

elements needed in small amounts = sodium, potassium, calcium, iron

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23
Q

organic growth factors are

A

molecules such as vitamins, essential amino acids

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24
Q

reactive oxygen species

A

aerobic cellular respiration or exposure to atmospheric oxygen leads to the formation

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25
Q

examples of ROS

A

hydrogen peroxide, superoxide, hydroxyl radicals

26
Q

superoxide dismutase

A

breaks down superoxide anions to produce hydrogen peroxide

27
Q

peroxidase

A

breaks down hydrogen peroxide to produce water; requires use of NADH

28
Q

catalase

A

breaks down hydrogen peroxide to produce water and oxygen; does not require use of NADH

29
Q

catalase test is done where

A

hydrogen peroxide is added to a smear of bacteria on glass slide

30
Q

positive catalase test

A

bubbling, bacteria has catalase

31
Q

negative catalase test

A

no bubbling, bacteria does not have catalase

32
Q

biofilms are

A

complex dynamic communities of bacteria (highly structured, and are there to provide selective advantage for members)

33
Q

formation of biofilms

A
  1. reversible attachment of planktonic cells
  2. first colonizers become irreversibly attachment
  3. growth and cell division
  4. production of EPS and formation of water channels
  5. attachment of secondary colonizers and dispersion of microbes to new sites
34
Q

problems with biofilms

A

medical devices (catheters) are suitable for biofilm formation, which can lead to deeper infections

hard to remove and difficult to treat with antibiotics and antimicrobial agents

35
Q

liquid media

A

broth cultures where cells are in suspension (grow large amounts)

36
Q

solid media

A

made by using agar (used to isolate for characterization)

37
Q

why agar is used in culturing

A

complex polysaccharide that is used as solidifying agent & not metabolized by many microbes

38
Q

chemically-defined media

A

used to grow microorganism where the specific requirements are known

39
Q

complex media

A

mixture of extracts and digesrs from yeasts, meats, or plants; used to grow a variety of microorganisms, or when sample identity is unknown

40
Q

selective media

A

a chemical has been added that allows for the growth for some microbes but prevents the growth of others

41
Q

differential media

A

a chemical has been added that allows identification of microbes based on their growth, colour or appearance on medium

42
Q

mannitol portion is differential because

A

we can visually see a pH change which indicates that fermentation has occurred

43
Q

salt portion is selective because

A

it only allows halophiles/halotolerants to grow

44
Q

blood agar is

A

differential media because it shows which colonies are capable of hemolysis

45
Q

beta-hemolysis
alpha-hemolysis
gamma-hemolysis

A

complete clearing
partial clearing
no clearing

46
Q

after one round of binary fission

A

the # of bacteria has doubled

47
Q

generation time is

A

the time it takes for a bacterial population to double

48
Q

lag phase

A

cell synthesizing new components either to replenish materials or adapt to new medium (# of cells changes very little because cells do not usually immediately reproduce in new medium)

49
Q

log/exponential phase

A

cellular reproduction is most active and generation time reaches a constant minimum

50
Q

stationary phase

A

growth rate slows and the number of microbial death balances the number of new cells (nutrients are being depleted while wastes accumulate)

51
Q

death phase

A

number of deaths increases and exceeds the number of new cells formed

52
Q

microscopic count is

A

using a microscope, a special slide is used that contains a grid and carries a known volume of sample, number of cells is then counted

53
Q

advantages of microscopic count

A

numbers can be achieved right away & method can be used for samples that do not grow well in labs

54
Q

disadvantages of microscopic count

A

overestimation in numbers as dead cells and debris can be counted as a cell; also cells that are moving may be counted multiple times

55
Q

turbidity

A

an indirect method where cells are counted by measuring the amount of turbidity (cloudiness)

56
Q

disadvantages of turbidity

A

standard curves are need as each species may scatter light differently

overestimation of bacterial numbers as dead cells and debris can be counted as a cells

57
Q

viable counts

A

the idea is to spread out the bacteria sufficiently so that each individual cell will result in a visible colony

of colonies = # of bacteria in the original sample

58
Q

viable counts measured in

A

colony-forming units

59
Q

goal to achieve a dilution to obtain a countable plate is

A

20-200 colonies

60
Q

steps for bacterial abundance in a sample

A
  1. calculate the bacterial concentration of usable dilution -> CFU/amount plated = CFU/mL
  2. calculate final dilution factor
    = total volume in tube/amount transferred (multiple all tubes together)
  3. calculate bacterial concentration of original sample
    bacterial concentration of the usable dilution X final dilution factor