2401 unit 5 Flashcards

1
Q

optimum growth temperature

A

the temp where growth rates are the highest

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2
Q

minimum growth temperature

A

the lowest temp in which the organism can still grow and replicate

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3
Q

max growth temp

A

the highest temp in which the organism can still grow and replicate

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4
Q

temp for psychrophiles

A

below 0 to 15

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5
Q

temp for psychrotrophs

A

4 - 25

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6
Q

temp for mesophiles

A

20 - 45

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7
Q

temp for thermophiles

A

50 - 80

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8
Q

temp for hyperthermophiles

A

80 - 100

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9
Q

optimal pH for acidophile

A

less than 5.5

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10
Q

optimal pH for neutrophile

A

~ 7

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11
Q

optimal pH for alkaliphile

A

8 - 11.5

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12
Q

hypotonic environments

A

lower concentration of solutes in the environment than the cell

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13
Q

hypertonic environments

A

higher concentration of solutes in the environment than the cell

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14
Q

for hypotonic, the movement of water into cell leads to

A

lysis

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15
Q

for hypertonic, the movement of water out of the cell leads to

A

crenation

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16
Q

microbes with cell wall are susceptible only to

A

hypertonic

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17
Q

hypotonic for cell wall microbes

A

water still moves into the cell; presence of the cell wall prevents lysis

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18
Q

hypertonic for cell wall microbes

A

movement of water out of the cell eventually leads to plasmolysis (separation of the plasma membrane from the cell wall)

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19
Q

halophiles

A

organisms that require salt for growth

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20
Q

halotolerants

A

organisms that do not need salt for growth but can survive and grow in the presence of high salt environments

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21
Q

macronutrients are

A

elements needed in large amounts = carbon, hydrogen, oxygen, phosphorus, sulfur and nitrogen

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22
Q

micronutrients are

A

elements needed in small amounts = sodium, potassium, calcium, iron

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23
Q

organic growth factors are

A

molecules such as vitamins, essential amino acids

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24
Q

reactive oxygen species

A

aerobic cellular respiration or exposure to atmospheric oxygen leads to the formation

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25
examples of ROS
hydrogen peroxide, superoxide, hydroxyl radicals
26
superoxide dismutase
breaks down superoxide anions to produce hydrogen peroxide
27
peroxidase
breaks down hydrogen peroxide to produce water; requires use of NADH
28
catalase
breaks down hydrogen peroxide to produce water and oxygen; does not require use of NADH
29
catalase test is done where
hydrogen peroxide is added to a smear of bacteria on glass slide
30
positive catalase test
bubbling, bacteria has catalase
31
negative catalase test
no bubbling, bacteria does not have catalase
32
biofilms are
complex dynamic communities of bacteria (highly structured, and are there to provide selective advantage for members)
33
formation of biofilms
1. reversible attachment of planktonic cells 2. first colonizers become irreversibly attachment 3. growth and cell division 4. production of EPS and formation of water channels 5. attachment of secondary colonizers and dispersion of microbes to new sites
34
problems with biofilms
medical devices (catheters) are suitable for biofilm formation, which can lead to deeper infections hard to remove and difficult to treat with antibiotics and antimicrobial agents
35
liquid media
broth cultures where cells are in suspension (grow large amounts)
36
solid media
made by using agar (used to isolate for characterization)
37
why agar is used in culturing
complex polysaccharide that is used as solidifying agent & not metabolized by many microbes
38
chemically-defined media
used to grow microorganism where the specific requirements are known
39
complex media
mixture of extracts and digesrs from yeasts, meats, or plants; used to grow a variety of microorganisms, or when sample identity is unknown
40
selective media
a chemical has been added that allows for the growth for some microbes but prevents the growth of others
41
differential media
a chemical has been added that allows identification of microbes based on their growth, colour or appearance on medium
42
mannitol portion is differential because
we can visually see a pH change which indicates that fermentation has occurred
43
salt portion is selective because
it only allows halophiles/halotolerants to grow
44
blood agar is
differential media because it shows which colonies are capable of hemolysis
45
beta-hemolysis alpha-hemolysis gamma-hemolysis
complete clearing partial clearing no clearing
46
after one round of binary fission
the # of bacteria has doubled
47
generation time is
the time it takes for a bacterial population to double
48
lag phase
cell synthesizing new components either to replenish materials or adapt to new medium (# of cells changes very little because cells do not usually immediately reproduce in new medium)
49
log/exponential phase
cellular reproduction is most active and generation time reaches a constant minimum
50
stationary phase
growth rate slows and the number of microbial death balances the number of new cells (nutrients are being depleted while wastes accumulate)
51
death phase
number of deaths increases and exceeds the number of new cells formed
52
microscopic count is
using a microscope, a special slide is used that contains a grid and carries a known volume of sample, number of cells is then counted
53
advantages of microscopic count
numbers can be achieved right away & method can be used for samples that do not grow well in labs
54
disadvantages of microscopic count
overestimation in numbers as dead cells and debris can be counted as a cell; also cells that are moving may be counted multiple times
55
turbidity
an indirect method where cells are counted by measuring the amount of turbidity (cloudiness)
56
disadvantages of turbidity
standard curves are need as each species may scatter light differently overestimation of bacterial numbers as dead cells and debris can be counted as a cells
57
viable counts
the idea is to spread out the bacteria sufficiently so that each individual cell will result in a visible colony of colonies = # of bacteria in the original sample
58
viable counts measured in
colony-forming units
59
goal to achieve a dilution to obtain a countable plate is
20-200 colonies
60
steps for bacterial abundance in a sample
1. calculate the bacterial concentration of usable dilution -> CFU/amount plated = CFU/mL 2. calculate final dilution factor = total volume in tube/amount transferred (multiple all tubes together) 3. calculate bacterial concentration of original sample bacterial concentration of the usable dilution X final dilution factor