Unit 1.2 - Cell structure and organisation Flashcards
What does cell theory state?
That all organisms are composed of cells
What are cells?
The basic unit of life
What can organisms be (out of two options)?
Unicellular or multicellular
Examples of unicellular organisms
-Amoeba
-Bacteria
Examples of multicellular organisms
-Plants
-Animals
What’s the first cell that all cells came from?
The zygote
What’s important about the zygote?
It’s the first cell that all cells came from
What is an embryo all made up of?
Undifferentiated stem cells
Name a cell that contains all undifferentiated stem cells
Embryo
What do new cells arise from?
Pre-existing cells
What do specialised cells arise from?
Undifferentiated stem cells
What type of cell arises from undifferentiated stem cells?
Specialised cells
What type of cell arises from pre-existing cells?
New cells
Specialised cell
A cell with a particular function in the organism
Stem cell
A cell that has the potential to divide into any kind of specialised cell
Give two examples + brief descriptions of specialised cells
-Muscle cells are made up of short fibres that shorten and contract to move the joints
-White blood cells destroy pathogens
What has led to us understanding the ultra structure of cells?
Advances in microscopy
What has advantages in microscopy led to us being able to do?
Understand the ultra structure of cells
Ultra structure of a cell
What’s inside it
What is the big recent advance in microscopy?
The electron microscope
Why is an electron microscope better than a light microscope?
Magnifies much more
Name two types of microscope in order of strength of magnification, starting with the weakest
-Light
-Electron
Disadvantage of the electron microscope
No colours
How does the electron microscope work?
Electron gun fires them out into a vacuum so that it molecules don’t effect them
Name four relevant units of measurement to this unit and how we transfer between them
metre (m)
| x1000
\/
millimetre (mm)
|x1000
\/
micrometre (weird-ńm)
|x1000
\/
nanometre (nm)
Which unit is most commonly used with cells?
The micrometre
What is the number used to transfer between units in this unit?
1000
(multiply or divide when relevant)
How many metres is one nanometre?
10^-9m
What type of scale can we use to measure from pretty big things to tiny things?
Logarithmic scale
What’s a logarithmic scale?
A way of displaying data over a wide range in a compact way
Out of those 13 items on the logarithmic scale, place them in order from smallest to largest
(Check your notes)
What are all cells surrounded by?
A membrane made of phospholipids and proteins (thin in biological membranes)
What is thin in biological membranes?
The membrane of phospholipids and proteins surrounding the cells
Name the two types of cells
Eukaryotic and Prokaryotic
What are the features of eukaryotic cells?
-Have a nucleus
-Have membrane bound organelles
-Can include plant and animal cells
What type of cells are plant and animal cells?
Eukaryotic
Organelles
A specialised structure with a specific function in a cell
(Enclosed in the cytoplasm)
What do membranes provide?
-A large surface area for the attachment of enzymes involved in metabolic processes
-A transport system inside a cell
What are the advantages of having membrane bound organelles?
-Potentially harmful chemicals like enzymes are isolated
-Molecules with particular functions (chlorophyll, for example) can be concentrated in one area
Size of an average animal cell
10-30 micrometres (weird mm)
What is cytosol?
Fluid contained within the cytoplasm
I physically cannot think how to do this logically on a flash card - please just find an unlabelled animal cell on the internet and learn all of the bits - I know. I’m sorry.
Can’t help you here
Find and label a generalised plant cell on the internet - I know. I wish I could make it go away.
Sorry love
Size of a generalised plant cell
10 - 100 micrometres (weird mm)
What is leucoplast?
Starch storage in plant cells
What does the cell wall do to a cell?
Prevents it from collapsing/swelling due to its water levels
What’s the site of photosynthesis in a plant cell?
Chloroplast
What’s the difference between plant and animal cells?
Plant cells have additional organelles and structures
E.g - chloroplasts for photosynthesis
-cellulose cell walls for support + to maintain turgor pressure
-Vacuole
What do plants’ cellulose cell walls do?
Support the plant cell and help maintain turgor pressure
What do prokaryotic cells have?
No membrane bound organelles
Which membrane bound organelles do prokaryotic cells not have?
-Nucleus
-Rough endoplasmic reticulum
-Golgi apparatus
-Mitochondria
-Chloroplasts
Give 2 examples of prokaryotic cells
Bacteria and archaea
Again, I’m sorry, but find and label a bacteria cell
Yep
What’s the size of a typical bacteria cell?
1-10 micrometres (weird mm)
What are 2 things that you label on the prokaryotic cell that’s unique to them?
Plasmids and mesosome
What are the things you’d label on a bacteria cell and not on the plants and animal cells?
-Flagellum
-Capsule
-Mesosome
-Nucleoid
-Pilus
-Plasmids
What does the nucleoid of a bacteria cell contain?
A long, circular DNA molecule
What type of cell wall does bacteria have?
Mucopolysaccharide (Murein)
What does the mesosome on a bacteria cell do?
Increases the surface area of the membrane to allow reactions to take place
+ For respiration
What’s the capsule on a bacteria cell for?
Formed of slime, to protect the cell from its environment
What does the Flagellum on a bacteria cell do?
Allows bacteria to move through its environment
Wha do the ribosomes on a bacteria cell do?
Manufacture Proteins inside the cell
What do the plasmids in a bacteria cell do?
As small bits of circular DNA, they carry genes that can be transferred from one cell to another
Compare Eukaryotic and Prokaryotic cells
(First listed are Eukaryotic cell features)
-Larger cells (10-100micrometres v.s 1-10micrometres)
-Ribosomes are larger and bound to the rough endoplasmic reticulum v.s smaller and free in the cytoplasm
-Membrane bound organelles v.s none
-DNA contained to the Nucleus v.s free in the cytoplasm
-Nucleus has a double membrane v.s no nuclear membrane/double envelope
-No plasmids v.s plasmids
-Cell wall (when present) is composed of Cellulose or Chitin v.s composed of pectidoglycan (mucopolysaccharide)
-Mitochondria are used for aerobic respiration, not a mesosome v.s no Mitochondria, uses a mesosome (a folded region of the cell membrane) for aerobic respiration
Which cells are largest?
Eukaryotic (10-100 v.s 1-10 micro metres)
Compare the ribosomes in Eukaryotic cells and Prokaryotic cells
Euk - Larger and bound to the rough endoplasmic reticulum
Pro - smaller and free in the cytoplasm
Compare the DNA in Eukaryotic and Prokaryotic cells
Euk - contained to the nucleus
Pro - free in the cytoplasm
Describe the nucleus in Eukaryotic v.s Prokaryotic cells
Euk - Has a double membrane
Pro - No nuclear envelope (double membrane)
Describe the differences in cell wall for the Eukaryotic and Prokaryotic cells
Euk - Cellulose or Chitin
Pro - Pectidoglycan (muccopolysaccharide)
Describe how Eukaryotic v.s Prokaryotic cells respire
Euk - Mitochondria are used (no mesosome)
Pro - No mitochondria, uses a mesosome (a folded region of the cell membrane)
What do Prokaryotic cells have that Eukaryotic cells don’t?
Plasmids and a mesosome
What do Eukaryotic cells have that Prokaryotic cells don’t?
Membrane bound organelles ( e.g - mitochondria)
What’s an appropriate way to describe a virus?
A complicated chemical
What are viruses to be referred to as?
Particles
Why are viruses not referred to as cells?
They don’t do the things that other living organisms do, like respire or reproduce
What kind of things do other living organisms do that viruses don’t?
Respire and reproduce
How do viruses reproduce?
Have to enter Prokaryotic/Eukaryotic cells and use their processes
What’s the average size of a virus particle?
0.1 micrometres (weird mm)
What are the three features to label on a virus particle?
-Nucleic acid (DNA/RNA)
-Surface proteins
-Protein coat (capsid)
What do the surface proteins of a virus particle help it do?
Gain entry into a host cell
Name for the protein coat of a virus particle
Capsid
What do viruses tend to be?
Very variable
Name 5 types of virus
-Adenovirus
-HIV
-Rabies virus
-Bacteriphage
-Influenza virus
Which type of virus is used in genetic engineering to transfer genes?
Adenovirus
What’s the Adenovirus used for?
Used in genetic engineering to transfer genes
What type virus can be transferred from mammals to humans? Why is this unusual?
The Rabies virus, as viruses usually only infect 1 species
Why is the Rabies virus unusual?
As it can be transferred from mammals to human and viruses usually only infect 1 species
Which virus infects bacteria?
Bacteriophage
What does bacteriophage do?
Infects bacteria
What do we know if the host cell is bacteria?
That the virus is bacteriophage
How will we know if the virus is bacteriophage?
The host cell is bacteria
Which type of virus causes the flu?
Influenza virus
What does the influenza virus do?
Causes the flu
How do viruses work?
- The viral genome enters the host cell and injects genetic material, instructing the cell to start making new viruses;
- Viral genome is replicated and transcribed - DNA is copied and used to make virus proteins (DNA - mRNA) : the virus has taken over the cell ;
- Viral mRNAs are translated and proteins processed - virus particles are assembled;
- Particles assemble inside the host, then burst or bud to exterior, destroying the cell - free particles in the tissue or Environment, ready to infect new cells
Which stage in how viruses work are Scientists still pretty uncertain about?
Part 3, where the virus mRNAs are translated and proteins processed (virus particles are assembled)
What type of process is viral replication?
Genetic
Functions of the nucleus
-Contains DNA (made in the ribosomes) which codes for/controls protein synthesis
-DNA replication occurs here
-Transcription produces mRNA templates
Size of the nucleus
4 micro metres
Draw and label a nucleus
(Check notes)
What is there linking the nuclear envelope and the rough endoplasmic reticulum?
Tubular continuity
What makes up the nuclear envelope of a nucleus?
Inner and outer nuclear membrane
Function of nuclear pores
Allow the transport of mRNA and ribosomes out of the nucleus
Function of nuclear envelopes/double membranes
Separates the contents of the nucleus from the cytoplasm
What’s another phrase for the nuclear envelope?
The double membrane
Chromatin function
Condenses before cell division to form chromosomes
How does chromatin work?
Wraps around histone protein molecules in the DNA as a way of organising the DNA in the nucleus. It can unwind to form proteins.
What type of protein does chromatin wrap itself around to organise the DNA in the nucleus?
Histone protein
Nucleolus function
Produces rRNA, tRNA and ribosomes
What is produced by the nucleolus?
-rRNA
-tRNA
-ribosomes
How do we recognise the nucleolus under the microscope?
Large dark patch
How does the rough endoplasmic reticulum get its name?
When seen under the electron microscope, the ribosomes on the surface can be seen
What IS the rough endoplasmic reticulum?
A system of parallel double membranes
Rough endoplasmic reticulum function
-Packaging and storing proteins (modifying them)
-Producing transport vesicles which merge to form the Golgi body
Draw and label the rough endoplasmic reticulum structure
(Check notes)
What do you call the gaps between the parallel rough endoplasmic reticulum membranes and what is their function?
Cisternae, which put the protein into 3D form
What do the cisternae do?
Put the protein into 3D form
What happens after a ribosome has made a protein?
It’s fed into the cisternae and fed throughout the cell
What makes the primary protein structure?
Ribosomes
How is a protein fed throughout a cell?
-Membranes arranged into sheets with cisternae in between
-Ribosomes make proteins
-Protein is fed into the cisternae
-Cisternae feeds the protein throughout the cell
Smooth endoplasmic reticulum function
Produce, package and transport steroids and lipids
What does the smooth endoplasmic reticulum NOT produce, package and transport?
Proteins
What does the smooth endoplasmic reticulum not have?
Ribosomes
What structures are present on the smooth endoplasmic reticulum?
-Membrane
-Cisternae (gaps)
What’s an accurate description of the Golgi body?
Very dynamic - lots happening
Functions of the Golgi body
-Packaging proteins for secretion from the cell
-Modification of proteins (e.g - adding carbohydrate chains to form glycoproteins)
-Producing lysosomes and digestive enzymes (tertiary structure)
Secretion
Where substances are discharged from the cell, in the example of the Golgi body, it’s proteins
What travels with the protein which it’s transporting to the Golgi body?
Vesicle
3 levels within the Golgi body that allows secretion to take place
-Cis cisterna
-Medial Golgi
-Trans cisterna
What does a vesicle do?
Travels and merges with the Golgi body with the protein that its transporting
What do vesicles fuse with?
The plasma membrane
What happens at the trans cisterna of the Golgi body?
-Proteins are modified - processed and packaged into vesicles, and parts bud off to form capsules that break off
-This ensures that proteins are delivered to the correct place
What do we mean when we say that proteins are ‘modified’ by the trans cisterna?
Processed and packaged into vesicles before breaking off as capsules
What are proteins synthesised by and where?
Ribosomes on the rough endoplasmic reticulum
How does the rough endoplasmic reticulum form vesicles?
Made of membrane, which can pinch off of the reticulum to form a vesicle
What forms a vesicle for secretion?
The rough endoplasmic reticulum
What do vesicles move towards by the Golgi body and why?
The cell membrane to release content
Which type of vesicles do not undergo secretion and why?
Some stay within the cytoplasm of the cell as they have specific functions here
Where do vesicles that do not undergo secretion stay?
In the cytoplasm of the cell
Ribosomes function
-Protein synthesis
-Primary protein structure is formed here
What type of protein structure is formed by ribosomes?
Primary protein structure
What do ribosomes consist of?
A small and a large subunit
How big are ribosomes?
About 10nm - incredibly small, more like big molecules
What can ribosomes be compared to and why?
Big molecules, as they’re so small (d= 10nm)
How does the ribosome know what protein to make?
-Instructions to make proteins are fed through, written on am mRNA molecule in the nucleus.
-Tells the ribosome, like a code, what type of protein to make
What are the instructions for protein synthesis written on for ribosomes?
mRNA molecules in the nucleus
What are mRNA molecules in the nucleus responsible for?
Being fed through the ribosomes, giving instructions of what type of proteins to make
Mitochondria function
ATP synthesis by aerobic respiration
What is the mitochondria vital for?
All cells that respire aerobically
What do cells respire anaerobically through?
The cytoplasm
What do cells respire aerobically through?
The mitochondria
Draw and label a mitochondria
(Check notes)
Where do lots of respiration reactions happen within the mitochondria?
In the matrix
Where is the site of ATP synthesis in the mitochondria?
Storked particles
What are storked particles in the mitochondria?
-Site of ATP synthesis
-Complex particles, imbedded in inner membrane
Where are storked particles to be found in the mitochondria?
Imbedded in the inner membrane
What are we seeing when we see the mitochondria through a microscope?
A thin slice through the organelle
What does the endsymbiont hypothesis state?
That mitochondria (and chloroplasts) may have been free-living, unicellular organisms millions of years ago
What’s the name of the hypothesis that states that mitochondria may have been free-living, unicellular organisms millions of years ago?
Endosymbiont hypothesis
What’s the proof for the endosymbiont hypothesis in the mitochondria?
-Circular DNA (similar to prokaryotic cells)
-Smaller ribosomes than the rough endoplasmic reticulum + cytoplasm (similar to prokaryotic cells)
-Fairly large (0.8 micro metres diameter)
How big are the mitochondria?
A diameter of around 0.8 micro metres
What type of cells does the mitochondria (and chloroplasts!) share similar factors with and what are they? What did this lead to (which is?)?
-Prokaryotic cells
-Circular DNA
-Smaller ribosomes than the rough endoplasmic reticulum + cytoplasm
=endosymbiont hypothesis
(The hypothesis that the mitochondria may have been free-living unicellular organisms millions of years ago)
What type of ribosomes does the mitochondria have?
Small, 70s ribosomes
What shape does the mitochondria have and why is this effective?
Cylindrical
-Larger surface area to volume ratio than a sphere
-Reduces diffusion distance between the edge and the centre
-Efficient aerobic respiration
What does the fact that the mitochondria is cylindrical mean for it?
Bigger surface area to volume ratio
-Reduces diffusion distance between the edge and centre for efficient aerobic respiration
What decreases and increases due to the mitochondria bing cylindrical in shape?
Decreases - Diffusion distance between the edge and the centre
Increases - Efficiency of aerobic respiration
What’s the distance between the edge and the centre of the mitochondria known as?
The diffusion distance
What deceases a mitochondria’s diffusion distance?
Being cylindrical in shape
What are eukaryotic cells usually doing if they divide?
Growing and replacing old cells
What are prokaryotic cells doing if they divide?
Creating entire new cells
Why is it important for plant cells to have their cellulose cell walls?
To maintain turgor pressure to avoid bursting
What do the lysosomes contain?
Powerful digestive enzymes to break down worn out organelles or cells
What happens to cells at the end of their lifespans?
Programmed cell death
How does programmed cell death work?
Cell produces many lysosomes, which burst open, release their enzymes and digest the whole cell
What actually ARE lysosomes?
Vesicles
What’s a vesicle?
Capsules formed from a membrane that contain something
What do the lysosome vesicles contain?
Digestive enzymes
What type of programmed cell death occurs with mutations?
Spontaneous
If programmed cell death happens spontaneously, what form does it take?
Mutations
2 examples of programmed cell death happening spontaneously in the form of mutations
-Metamorphosis- tails of tadpoles are broken down
-Embryos fingers are released when a membrane is broken down
What do the digestive enzymes in lysosomes do?
Break down worn out organelles or cells
What can be done with worn out organelles?
Digested by the powerful digestive enzymes of lysosomes and can be re-used
Where do vesicles form and how does this form a lysosome?
Vesicles form from the Golgi body and bud off to form a lysosome
How do phagocytes use lysosomes?
To digest engulfed bacteria
What do phagocytes use to digest engulfed bacteria?
Lysosomes
What do centrioles do?
Form the spindle during cell division
What does the spindle of a centriole do?
Connect chromosomes and pull them apart
What connects to chromosomes and pulls them apart in centrioles?
Spindle