Unit 1 Vocab Flashcards
microorganism
life forms too small to be seen by the eye
microbial ecology
study of how microbes affect animal, plants, and entire global ecosystems
human microbiome
environment of microbes living naturally inside humans affecting their health
DNA
genetic information
ribosomes
synthesize proteins
cytosol/cytoplasm
aqueous mixture of macromolecules, small organics, ions, and ribosomes inside cells
plasma membrane
selectively permeable layer composed of phospholipids and proteins
prokaryotes
unicellular organisms that loack a distinct nucleus and membrane-bound organelles; characterized by simple structure and genetic information in the cytoplasm
eukaryotes
organisms whose cells have a true nucleus and mombrane-bound organelles; typically have more complex structure
bacteria
single-celled microorganisms that lack distinct nucleus and mombrane-bound organelles; diverse shapes and sizes
peptidoglycan
a structural polymer consisting of sugars and amino acids that forms the rigid cell wall in bacterial cells; provides strength and shape
Gram-negative
bacteria that is resistant to gram-staining method; typically have an outer membrane and less peptidoglycan
Gram-positive
bacteria with a thick layer of peptioglycan, making them subjects of gram-staining method
archaea
group of single-celled microorganisms that, like bacteria, lack a true nucleus and membrane-bound organelles, but they differ significantly in genetic and biochemical characteristics, often thriving in extreme environments.
eukarya
one of the three domains of life, encompassing organisms with complex, membrane-bound organelles and a true nucleus in their cells, including plants, animals, fungi, and protists.
fungi
can be single or multicellular eukaryotes
chitin and glucan
carbohydrates that make up cell walls of fungi
zoosporic fungi
free living, often motile
yeasts fungi
unicellular; include human microbiota, primary pathogens, and opportunistic pathogens; some reproduce by budding; some reproduce by spore formation
dimorphic fungi
have yeast-like and mold-like forms; include oppotunistic pathogens
mold fungi
multicellular
mushroom fungi
multicellular with macroscopic fruiting bodies
acellular entities
things not composed of cells
viruses
acellular; obligate parasites that only replicate within host cells; include DNA or RNA and a protein coat called a capsid
viroids
acellular; composed of RNA
satellites
acellular; composed of nucleic acid enclosed in a protein shell
prions
acellular; composed of proteins
ribozymes
RNA molecules; most likely probiont molecule
endosymbiosis
interaction between two organsism in which one lives inside of another
phylogeny
relationships among organisms determined by rRNA, genome sequence, cellular characteristics and/or metabolic pathways
taxonomy
the classification system used to group organisms
binomial nomenclature
how microorganism are named; Ex. Genus species
microscopy
the technique and science of using microscopes to observe and study objects
culture
cells grown in/on nutrient media
pure culture
isolated strain
colony
visible, billions of cells, pure culture
medium
liquid/solid mixture containing required nutrients for growth
growth
increase in cell number resulting in cell division
genome sequencing
only about 1% of microbes are culturable so genomes tell us what an unculturable microbe might be capable of (metagenomics)
robert hooke
best known for the first observations of cells; became popular because of beautiful illustrations of fleas and fruiting structures of molds
antoni van leeuwenhoek
first to describe what we now know were bacteria and protists (animalcules)
spontaneous generation
long held theory that microbes can arise spontaneous from nonliving matter
louis pasteur
- develops swan neck flask that allows air within, but traps microbes from air
- demonstrates that microbes from air can cause “contamination” and confirms that heating can remove microbes
Koch’s postulate
- the microorganism must be present in every case of the disease but absent from healthy organisms
- the suspected microorganisms must be isolated and grown in a pure culture
- the same disease must result when the isolated microorganism is inoculated into a healthy host
- the same microorganism must be isolated again from the diseased host
immunity
protection from infection due to previous exposure to a pathogen
jenner’s experiment
discovery of small pox vaccine due to previous exposure to cowpox
microbial diversity
focuses on nonmedical aspects and metabolic processes of microbes in soil and water
magnification
ability to enlarge an image
resolution
the ability to distinguish two adjacent objects as distinct and separate
lenses
create images by bending light
focal point
specifc location where parallel rays of light converge
oil immersion objective
100X objective lens with a vey short working distance
dyes
used to increase contrast
gram staining
differentiates gram-negative and gram-positive bacteria
acid-fast staining
differentiates bacteria that have mycolic acid in their cell wall from those don’t
cell envelope
layers surrounding a cell
bacterial phospholipids
fatty acids are attached to glycerol by ester linkages
archaeal phospholipids
attached to glycerol by ether linkages
uniport
move a specific molecule against the concentration gradient