Unit 1 Section 1: Studying cells- Microscopes

Question 1

Define magnification

Answer 1

How much bigger the image is than the sample(specimen)

Question 2

Define Resolution

Answer 2

How detailed the image is, it is the ability of a microscope to distinguish between to points that are close together

Question 3

sum for magnification

Answer 3

magnification = length of image / length of specimen

Question 4

two types of microscope

Answer 4

Electron

Light

Question 5

two types of electron microscope

Answer 5

Scanning (SEM)

Transmission (TEM)

Question 6

Advantages/Disadvantages of TEM

Answer 6

Advatages- Provide high resolution images

Disadvantages- Specimen need to be sliced thinly.

Question 7

Advantages/Disadvantages of SEM

Answer 7

Advantages - Show the surface of the specimen and can be in 3D

Disadvantages - Lower Resolution than TEM

Question 8

Max Resolutions

Answer 8

Light microscope - 0.2um
TEM - 0.0001um
SEM- 0.005um

lower resolution the better

Question 9

Max Magnification

Answer 9

Light microscope - x1500
TEM - more than x1,000,000
SEM- less than x1,000,000

higher magnification the better

Question 10

dye examples

Answer 10

methylene blue- stains RNA/DNA

eosin - highlights cell membrane

Question 11

Electron micrograph images

Answer 11

are always black and white (but sometimes colour is added artificially to images don’t confuse this with staining)