Trypanosoma

Question 1

Sample and method

Answer 1

Blood smear- 3 slides

Giemsa staining

Question 2

For diagnosing, what forms do you find in the vector/insect?

Answer 2

Promastigote or epimastigote

Question 3

For diagnosis, what forms do you find in the vertebrate/reservoir?

Answer 3

Amastigote- tissues

Metacyclic trypomastigote- this is the infective form

Question 4

Type of development

Answer 4

Direct or indirect

A-pro-epi-tryp

Question 5

Type of spreading

Answer 5

Biological: cyclic
Mechanical: non-cyclic

Question 6

General morph

Answer 6

Monomorph or pleomorphic 
10-14 micrometres
Large nucleus
Kinetoplast
Flagellum with undulating membrane 
Unicellular

Question 7

Which are monomorphic?

Answer 7

Vivax and congolense

Question 8

Which are pleomorphic?

Answer 8

Brucei

Question 9

What are the diseases caused?

Answer 9

Nagana
Dourine/trypanosoma equiperdum
Surra

Question 10

Nagana?

Answer 10

vivax
congolense
brucei brucei

Question 11

Surra

Answer 11

brucei evansi

Question 12

Dourine

Answer 12

brucei evansi

brucei equiperdum

Question 13

Vector of Nagana?

Answer 13

Both sexes of tsetse flies

Question 14

Vector of surra?

Answer 14

Tabanid/horse fly
Capybara
Vampire bats

Question 15

Vector of T.cruzi?

Answer 15

Assasin/kissing bug

Question 16

Vector of dourine?

Answer 16

has no vector!

Veneral transmission

Question 17

Clinical signs of Nagana

Answer 17

Lymphoid enlargement
Splenomegaly
Horses- edema of genitals/legs
Dog/cat: myocarditis

Question 18

Clinical signs of dourine

Answer 18

Genital mm 
Ventral abd edema 
Hypopigmentation
Dollar spots- urticaria 
Nerves

Question 19

Clinical signs of Surra

Answer 19

“disease of the hip”
Posterior paralysis in the horse
Edema
Ocular signs

Question 20

T. cruzi

Answer 20

Crescent shaped 
Pseudocysts in cardiac muscle 
Vectors love the skin around the eye
Get out with faeces 
Penetrate skin by scratching, get in by blood sucking