Transfusion

Question 1

minimum criteria to donate blood

Answer 1

Hb 135g/L in men
Hb 125g/L in women
50kg weight
questionnaire

Question 2

what happens to the bag of donated blood?

Answer 2

centrifuged to separate components and samples undergo microbiological testing

Question 3

what microbiological testing is taken on blood donation?

Answer 3

HIV
hep B
hep C
HTLV
syphilis

Question 4

storage of red cells

Answer 4

4 degrees for 35 days

Question 5

storage of FFP

Answer 5

-30 degrees for 3 years

Question 6

storage of platelets

Answer 6

stored at room temperature (22 degrees) for 7 days with agitation (shaken)

Question 7

blood components available

Answer 7

red cells
FFP
platelets
cryoprecipitate (fibrinogen)

Question 8

blood products available

Answer 8

anti-D

prothrombin complex concentrate

Question 9

what chromosome is the ABO system found on?

Answer 9

chromosome 9 (ABO gene)

Question 10

which alleles are dominant in the ABO blood grouping system?

Answer 10

A and B are co-dominant, O is silent

Question 11

population frequency of the blood types

Answer 11

group O= 47%
group A= 42%
group B= 8%
group AB= 3%

Question 12

genotypes of the blood groups

Answer 12

group O= OO
group A= AA or AO
group B= BB or BO
group AB= AB

Question 13

what is Landsteiner’s Law?

Answer 13

when an individual lacks A or B antigen, the corresponding Ab is produced in their plasma

Question 14

population frequency of Rh +ve

Answer 14

85%

Question 15

which allele is dominant in the rhesus system?

Answer 15

D is dominant (+ve)

Question 16

genotype of RhD status

Answer 16

RhD +ve= DD or Dd

RhD -ve= dd

Question 17

what can the D Ab cause?

Answer 17

transfusion reactions

haemolytic disease of the foetus/ new-born

Question 18

what is a cross-match?

Answer 18

checks donor cells are compatible with patient plasma

Question 19

indications for FFP (clotting factors)

Answer 19

bleeding in patient with coagulopathy
prophylaxis prior to surgery/ procedure with coagulopathy
massive haemorrhage
transfuse early in trauma

Question 20

monitoring of patient given transfusion

Answer 20

before
15 minutes
within 60 minutes of completion