Transformation efficiency

Question 1

Formula

Answer 1

of colonies/Amount of DNA plated

Question 2

Calculate TE if 100 colonies grew on a plate, with .1 mL of DNA added.

Answer 2

1 x 10^3 (or 1000)

Question 3

Calculate TE if 30 colonies grew on a plate, with .5 mL of DNA added.

Answer 3

6 x 10^1 (or 60)

Question 4

Calculate TE if 250 colonies grew on a plate, with .4 mL of DNA added.

Answer 4

6.25 x 10^2 (or 625)

Question 5

Calculate TE if 5 colonies grew on a plate, with .01 mL of DNA added.

Answer 5

5 x 10^2 (or 500)

Question 6

Calculate TE if 90 colonies grew on a plate, with .3 mL of DNA added.

Answer 6

3 x 10^2 (300)