SDS Page Flashcards
1
Q
What does SDS page stand for?
A
Sodium Dodecylsulfate Polyacrylamide Gel Electrophoresis
2
Q
What parts does SDS page have?
A
Stacking and resolving gel
3
Q
What is the purpose of a stacking gel?
A
To hold samples before sorting
4
Q
What is the purpose of a resolving gel?
A
To display and allow samples to seperate
5
Q
Do small or large proteins move faster?
A
Small
6
Q
What does SDS do?
A
Gives proteins negative charge and breaks disulfide bond
7
Q
What does the speed of migration depend on?
A
Dimension, form and charge of proteins
8
Q
How do you stain a protein gel?
A
Coomassie brilliant blue stain
9
Q
How can you differentiate between proteins of the same size?
A
Use antibodies by doing a Western Blot
