Chromatography types

Question 1

Size Chromatography

Answer 1

Separate by the size of the proteins
Pores in the beads catch the smaller proteins (take longer) allowing the larger proteins to move faster through the column

Question 2

Affinity Chromatography

Answer 2

EG Nickel, binds to His-6 tags on proteub due to affinity

Question 3

Why is Imidazole used in nickel affinity chromatography?

Answer 3

To release proteins from nickel because it binds more tightly to nickel than his-6

Question 4

Charge Chromatography

Answer 4

Beads are 1 charge, attract opposite charge

Question 5

In Charge chromatography, beads that are the same charge as the bead _________ the column

Answer 5

flow through

Question 6

Hydrophobicity Chromatography

Answer 6

Hydrophobic beads cause other hydrophobic proteins to stick because they are avoiding water

Question 7

Salt buffers with release _________ before the _____________

Answer 7

less hydrophobic proteins, hydrophobic proteins