Transfer of Bacteria: Aseptic Technique

Question 1

what is a contaminant?

Answer 1

a polluting or poisonous substance that makes something impure

Question 2

Aseptic technique?

Answer 2

used in microbiology to exclude contaminants

the purpose is to prevent contaminiation

Question 3

sterile / sterilization

Answer 3

all culture medias which are used to grow microorganisms are sterilized in which they are rendered free of all life

Question 4

Broth culture?

Answer 4

liquid mixture that provides large numbers of bacteria in a small space and are easily transported

Question 5

agar slants

Answer 5

are test tubes containing solid culture media that were left at an angle while the agar solidified

Question 6

agar deep

Answer 6

agar is allowed to solidify in the bottom of a test tube

Question 7

what is the importance of agar deep?

Answer 7

they are used to grow bacteria that require less oxygen than is present on the surface of the medium.

Question 8

Aseptic inoculation technique from broth to slant?

Answer 8

Sterilize Loop 
with other hand take cap off and hold tube at an angle 
then sterilize the top of the tube 
get a loopful of culture
heat the mouth of the tube, replace cap

Question 9

Deep and broth

Answer 9

sterilize needle with other hand take cap off and hold tube at an angle
then sterilize the top of the tube
stab agar deep with needle
heat the mouth of the tube, replace cap

Question 10

slant to slant

Answer 10

inoculate slant by streaking the loop back and forth across the surface of the agar

Question 11

What is the purpose of flaming a loop and why must it be cooled before collecting organisms?

Answer 11

to sterlize and kill an bacteria and avoid contamination

you must wait for it to be cooled so that it wont kill off what you are trying to inoculate.