Bacteria of the Resp. tract and G.I

Question 1

Bacteria of Resp Tract: Blood agar

how do you identify Alpha hemolysis?

Answer 1

Greenish color around colonies

* think of alphas punching and leaving bruises

Question 2

Bacteria of Resp Tract: Blood agar

identify beta?

Answer 2

complete loss of color you will see clearing zone around bacteria

Question 3

Bacteria of Resp Tract: Blood agar

gamma hemolysis?

Answer 3

no change to blood agar just growth

Question 4

Bacteria of Resp Tract: Blood agar

what is the enzyme responsible for beta hemolysis

Answer 4

hemolysin

Question 5

Bacteria of Resp Tract: Blood agar

which group of streptococcus are sensitive to the antibiotic bacitracin

Answer 5

Group A streps

Question 6

MacConkey Agar:

what does it identify?

Answer 6

gram-negative organisms

Question 7

MacConkey Agar:

what makes this media selective?

Answer 7

Bile salts

Question 8

MacConkey Agar:

what is the compound this media differentiates?

Answer 8

Lactose

Question 9

MacConkey Agar:

which family of bacteria does this media use to identify?

Answer 9

enterobacteriaceae

Question 10

MacConkey Agar:

lactose fermenting colonies will be what color?

Answer 10

pink or have a hint of pink

Question 11

MacConkey Agar:

non lactose fermenters?

Answer 11

colorless colonies

Question 12

TSI AGAR: Triple sugar iron agar

if it shows fermentation of 2 or more sugars it looks?

Answer 12

all yellow

Question 13

TSI AGAR: Triple sugar iron agar

if it shows fermenation of one sugar?

Answer 13

the butt will be yellow

Question 14

TSI AGAR: Triple sugar iron agar

if gas is produced you will see?

Answer 14

gas bubbles

Question 15

TSI AGAR: Triple sugar iron agar

if h2s is produced you will see?

Answer 15

black bottom

Question 16

Rapid Strep Test:

How do you identify a postive test?

Answer 16

pink line down the bottom

Question 17

Rapid Strep Test:

serological immunoassay?

Answer 17

to test for antigen