Protein catabolism Flashcards
Gelatin hydrolysis: positive test result?
Negative test result?
If it is liquid
not liquid
Gelatin hydrolysis: reactant, enzyme, and product?
Gelatine, gelatinase, Amino acids
Gelatin hydrolysis: why is it necessary to chill the tubea prior to interpreting ?
to prevent false positives
Urea Hydrolysis: positive test? negative test?
positive if hot pink because of ammonia
negative if yellow
Urea Hydrolysis: reactant enzyme and product?
urea
urease
ammonia
Urea Hydrolysis
ph if postive?
ph if negative?
if positive it will be alkaline
if negative acidic
Phenylalanine Media:
positive reaction?
negative reaction?
positive if dark green
negative if light green
Phenylalanine Media:
what is this experiment doing?
deamination in which an amino group is removed
Phenylalanine Media:
what is the reagent used to demonstrate that reaction?
which can be seen by it turning a dark green color bc of the FERRIC IRON
Ornithine Decarboxylation:
a positive test?
negative test?
positive test results in a PURPLE COLOR
negative test results in a YELLOW COLOR
Ornithine Decarboxylation:
what is this experiment demonstrating ?
decarboxylation showing that the amino acid ornithine is being used by the microbe for energy and growth
SIM TEST:AKA INDOLE TEST
postive indole test?
negative indole test?
pink if postive
yellow if negative
SIM TEST:AKA INDOLE TEST
substrate, enzyme, product ?
tryptophan, tryptophanase, indole
SIM TEST:AKA INDOLE TEST
what was the reagent added?
Kovacs
SIM TEST:AKA INDOLE TEST
how do you identify motilty ?
you get cloudyness
